Studies On Tissue Culture And Plant Regeneration Of Euphorbia Cotinifolia Linn.

The study was mainly to probe an efficient way through tissue culture and rapid propagation of Euphorbia cotinifolia Linn.. The leaves, stems or shoots of young branches was taken as explants. The tissue culture, somatic embryogenesis and plant regeneration of Euphorbia cotinifolia Linn. were systematically studied. The main results as below:1. Different treatments on explants were taken during initial culture as follows: Cutting the leaves into 1.0 cm~2 was an appropriate way, and dark treatment for 7 days was the best way for decreasing browning rate. The browning rates were 28.9% and 38.0% respectively.2. Starting of shoots and stems: The optional culture medium for their initiation was MS with 6-BA 1.0 mg/L and NAA 0.1 mg/L. The initial rate was 71.4%.3. Induction of callus from leaves: Callus was induced in MS medium with 6-BA 0.5 mg/L and NAA 0.1 mg/L. The induction rate could up to 57.2%.4. Proliferating and differentiating cultivation: The optional proliferating buds culture medium from stems and shoots was MS supplemented with 6-BA 1.0 mg/L, IBA 0.5mg/L and NAA 0.1 mg/L, on which it could multiply 5.56 times after 30 d. Highly proliferating callus was obtained from the leaves following cultivation on MS medium supplemented with 6-BA 0.5 mg/L, 2,4-D 0.1 mg/L and NAA 0.5 mg/L. The proliferating rates could be up to 86.4%. The best culture medium for callus buds differentiating was MS with 6-BA 1.0 mg/L and NAA 0.1 mg/L. The regeneration rate was 71.4%.5. Rooting culture: Regenerated shoots could produce roots in 1/2 MS medium...