Studies On Biology Of Pollination Fertilization And Embryo Aborted In Wuhexuegan (C.sinensis (L.) Osbeck.)

The development condition of Wuhexuegan embryos is closely related with seed development. It was approved that the seedlessness of Wuhexuegan was stable, but the research on biological character and embryo development in it was poor, it has great value to study the abortive embryo in Wuhexuegan. This thesis worked on the physiological and molecular biology of Wuhexuegan in the aspects of pollination, fertilization, embryo development and specific proteins et al. It has great theoretical and practical significance to study the physiological and biological mechanism of embryo development in Wuhexuegan, which will help to find out the expressive law from gene to character, select Xuegan varieties, control fruit seeds and so on.1 Biology of pollination fertilization in Wuhexuegan and XuganThe morphological character of flower, the pollen germination both in vitro and at the stigma were studied in the experiment. It showed great differences at the pollen germination rate of the three combinations, the rate of Wuehexuegan was lower than that of Xuegan, it was 9.33% and 26.91% in vitro respectively. At the same time, the pollen germination rate of Xuegan × Xuegan was the highest among the three pollination combinations, at the rate of 80%. But that of Wuehexuegan × Wuehexuegan was the lowest, only 5%. The length of Xuegan's pollen tube was about 3-5 times of pollen grain diameter, it was the longest too. So did the fruit setting rate. The poor papillae cell and stylar canal of stigma of Wuhexuegan, self-incompatiblility resulted in the low fruit setting rate. The fruit setting rate of Xuegan × Xuegan was 1.91 %, while that of Wuehexuegan × Wuehexuegan and Wuehexuegan × Xuegan were respectively 0.25% and 0.38%.