Breeding Research On Blast-resistance Gene And Insect-resistance Gene In Rice

Taking super rice restoring lines: Bpi9, C60, C461, C384, C659, R207, M86,9311, Kefeng6hao, hybrid rice IIyou838 and so on, which contain Pi9 gene or BT gene as experimental materials, molecular assay had been done and agronomic traits had been assessed. The conclusions were drawn out as follow:1. By PCR analysis, screened 22 hills from 103 materials which contain Pi9 gene.2. By PCR analysis and BT genetic test paper, the genome of these plants measured not only contain BT gene, but also the BT gene could be expressed normally.3. Though PCR analysis the DNA of 5 lines which could contained Pi9 gene and BT gene. The genome of the 5 lines contain BT gene, but only 2 lines contain Pi9 gene4. Field resisting qualification that the plant resisting the rice blast to the molecule mark carried on the rice blast, in the field resisting qualification, the genome of these plants with Pi9 gene could be produced the resisting to the rice blast, but there was one hill among them, it could be detected by PCR analysis, but it could not resist the Magnaporthe grisea infection when it was planted in field. The reason of that the blast-resistance gene could be lost.5. Field evaluations were conducted to investigate the resistance of BT transgenic rice. The result indicated that these transgenic rice lines inserted with the combination of BT were highly resistant against Chilo suppressalis and Cnaphalocrocis medinalis. Field evaluations were conducted to investigate the resistance of BT transgenic rice. The result indicated that these transgenic rice lines inserted with the combination of BT were highly resistant against Chilo suppressalis and Cnaphalocrocis medinalis. Compared with CK the damage rate of the Chilo suppressalis has dropped by 80% to 100%, the damage rate of the Cnaphalocrocis medinalis has dropped by 21% to 66.75%.