The Analysis Of Tripenoids Of The Fruit Of Chinese Jujube

The ursolic acid and oleandic acid of the fruit of Chinese jujube varieties Jinsixiaozao and Dongzao were separated and analyzed through the whole fruit development season by reversed-phase high-performance liquid chromatographic (HPLC) with optimized conditions established for Chinese jujube for the first time. The proper extraction process of tripenoids from jujube was established too. Ursolic acid, oleandic acid, prandial fibre and total flavone, four kinds of functional components, of more than 60 jujube varieties were measured and compared. Meanwhile the indexes of the liquid medicine "Date" were detected. The main results are as follows:1. The proper HPCL conditions for ursolic acid and oleandic acid measurement: HYPERSIR C₁8 BDS (250mm*4.6mm, 5μm), 18℃, UV λ 210, 0.6ml/min, solid phase methnol: water(92:8), 300ppm phosphate, sample 55 μ m.2. The changing of ursolic acid and oleandic acid of classified fruit collected at the same day of different fruit diameter: Before August, there is no difference of the contents of these two acids in Jinsixiaozao of different size, and in Dongzao the ursolic acid decreases as the diameter of jujube increases and the oleandic acid is a bit little. After August, these two acids decreases as the diameter of Jinsixiaozao increases;it is opposite in Dongzao. So the changing rule of ursolic acid and oleandic acid of Jinsixiaozao and Dongzao in the period of fruit growth is as follow: the average contents of these acids increase with the growing of Jinsixiaozao until August 11th they began to decrease. The average contents of these acids increases with the growing of Dongzao.3. Ursolic acid, oleandic acid, prandial fibre and total flavone of jujube varieties were measured, with the highest ursolic acid and oleandic acid(728.9038 μ g/mL) in Yucituanzao, prandial fibre (9.0418%) in Mopanzao, and total flavone(7.036mg/mL) in Lingzao.4. Comparing the four kinds of triterpenoids extraction methods, including soxhlet, thermal refluxing, petroleum ether and ultrasonic, ultrasonic is the best way. Among methanol, ethanol, water and petroleum ether, methanol is the best solvent. The proper extraction conditions for ursolic acid were as follows: 95%methanol, l:10(m/v) for the ratio of material to solvent, extraction for three times, each time 15 minutes. The proper extraction conditions for oleandic acid were as follows: 95%methanol, l:10(m/v) for the ratio of material to solvent, extraction for two times, each time 15 minutes. The optimized ultrasonic extraction conditions for triterpenoids were as following: 85% alcohol, l:15(m/v) for the ratio of material to solvent, extraction for four times, each time 15 minutes.