Inhibition Of Cupric Complexes Of Amino Acid On Growth And Phytotoxic Protein Of Verticillium Dahliae

Verticillium wilt of cotton plant which infected by Verticillium dahliae is a kind of plant fungal disease in the vascular bundle. Verticillium dahliae can form multicelled, melanized, resting bodies called microsclerotia which persist in the soil for many years. The microsclerotia are tiny and black. It can even form on and in the fine roots of many species of resistant plants without causing symptoms. so Verticillium dahliae can cause the cotton wilt in several years. The cotton wilt cause enormous loss of the quantity and quality of cotton.Now we are trying to use diverse ways to control the disease.In our laboratory, cupric complexes of amino acid are tested to manage the Verticillium dahliae. In this research, the ultraviolet spectroscopy was used to determinate the coordination matching of the cupric complexes of mixed amino acid. To identify the reliability of the result, we use the same way to test the coordination matching of these eight ingredients, at the same time, the background absorbance of the amino acid has no effect on the absorption of the complexes, the results show that the cupric amino acid complexes has the absorption at 230nm, the ultraviolet spectroscopy can be used to test this coordination matching and the matching is 2:1, and the NaOH titration analysis showed that copper ion bonds the carboxyl of amino acid, then it attests this coordination matching at the side of the chemical structure. This research provides a new practical way to prepare the cupric complexes of mixed amino acid.The cupric complexes of Alanine, cupric complexes of Glycine, cupric complexes of Glutamic Acid, cupric complexes of Threonine and cupric complexes of mixed amino acid were tested to inhibit the Verticillium dahliae, the results showed that the four fungicides all inhibited the growth of the microsclerotia, postponed its formation, and decreased the melain formation in the microsclerotia. By comparison, it was concluded that cupric complexes of Alanine and cupric complexes of Glutamic Acid were apparently better than the others.We used cupric complexes of Alanine, cupric complexes of Glycine, cupric complexes of Glutamic Acid, cupric complexes of Threonine and cupric complexes of mixed amino acid to test the inhibition on the secreted protein from Verticillium dahlia. Verticillium dahliae was cultured in the Czapeck's culture solution which added the cupric complexes of amino acid at 25 ℃, and the control added aseptic water. After 20 days we extracted the secreted protein of...