Study On Inducing And Culturing Of Bulblet From Flower Organ Of Lilium Spp.

Lily (Lilium spp.) belongs to lily family (Liliaceae) and is perennial herb with underground bulb. Lily plant is one of worldwide famous flowers and grown in domestic and foreign botanical gardens. Lily is suitable for potting, fresh cut flower, and garden combination. It can also be used as medicinal purposes and edible food. Many researches have been done on lily tissue culture in domestic and abroad. However, most baby bulb is abducted by squama explant. Test tube bulb diameter is small and survival rate of transplanting is low, which restricts reproduction rate and commercial production. Additionally, research on bulblet induction from Lilium star Gazer and Lilium trompeteni organ has not yet been reported. Therefore, this experiment is to seek method for bulb induction, increase bulb thickness, and improve large-scale production of Lilium trompeteni and Lilium star Gazer. Effects of different basic media and different concentration of hormone on induction and proliferation of bulblet from ovary, style, and filament of Lilium Star Gazer and Lilium trompeteni were tested in this experiment. The results were reported as below:1. The bulblet can be induced both from flower organs of Lilium Star Gazer and Lilium trompeteni, but the capacity of bulblet induction was better from Lilium Star Gazer than from Lilium trompeteni.2. Bulblet induction capacity was different from different part of the same species. Bulblet induction rate from ovary was the highest, followed by style, the lowest was from filament of Lilium Star Gazer, while in Lilium trompeteni, the highest bulblet induction from filament, followed by style, and the lowest was from ovary. The bulblet induction rate can be at 66% from ovary of Lilium Star Gazer, which was the highest among all.3. Different basic media had different effects on bulblet induction, B₅ media was the best, followed by MS, N₆ had the lowest induction rate. MS media had the best proliferation effect on Lilium trompeteni, followed by B5, and the lowest was N₆.4. Different concentration of 6-BA had effect on bulblet induction in different Lilium species. The best 6-BA concentration for bulblet induction from ovary was at l.Omg/L for Lilium Star Gazer, while the best 6-BA concentration for bulblet induction from ovary was at 0.5 mg/L for Lilium trompeteni. The result showed effect of different 6-BA concentration on bulb proliferation. As 6-BA concentration increasing from 0.5 to 2.0mg/L, bulblet proliferation rate increased. The best 6-BA concentration was at 2.0mg/L.5. The lower NAA concentration increased bulblet induction rate in ovary culture of Lilium Star Gazer. The highest proliferation rate showed on NAA concentration 0.2mg/L, proliferation rate was 88.55%. When NAA concentration was lower than O.lmg/L, or higher than 0.6mg/L, bulblet proliferation rate was not good.6. The best culture medium for bulb induction from ovary of Lilium trompeteni was N6 + 6-BA0.5¹.0mg/L + NAA0.1⁰.2mg/L. The best culture medium for bulb induction from ovary of Lilium star Gazer was N6 + 6-BA0.5².0mg/L + NAA0.02⁰.05mg/L. The best culture medium for ovary buld proliferation from Lilium trompeteni was MS + 6-BA1.0 ².0mg/L + NAA0.1⁰.2mg/L.