Study On The Overexpression Of HbNHX1 Gene From The Hordeum Brevisubulatum In Rice

Soil salinization is a serious problem in agriculture, which affects the agricultural production and ecological environments, and leads to a great reduction output inrice. The total acreage of salinizes and alkaline land is about 1/3 of the irrigated land in the world. In our country the salt disperative soil is about 1/5 of arable land and it will be expanded in the future. The production loss caused by soil salinization is too large to be estimated. In addition, decrease of arable land and shortage of freshwater will compelled people to exploit and utilize wast saline and coastal soil. Therefore, the research on the mechanism of plant salt-tolerance and breeding for salt-tolerant crops will become an attention rsearch field.Compartmentalization of Na~+ in vacuole is an important mechanism for plant, especially halophyte, to reduce the toxicity of Na~+. The vacuolar Na~+/H~+ antiporter plays an important role in Na~+ compartmentation. To further investigate the function of HbNHXl, we also overexpressed HbNHXl in rice by gene transformation via Agrobacterium. PCR, PCR-Southern and RT-PCR analyses of transgenic plants showed that the HbNHXl gene was integrated into the rice genome and expressed at the transcriptional level. This work will be helpful to abatain the transgenic rice which can grow better under abiotic stresses.