Testing And Rapd Markers Of Blumeria Graminis F.SP. Tritici Isolates To Triadimefon

The sensitivities of Blumeria graminis f.sp. tritici to triadimefon were identified and the isolates,which were collected from Beijing,Henan , Hebei, Sandong, Gansu and Sichuan were test. The results showed that the lowest EC50 of isolates collected from Beijing was 7.7318μg/mL, which of the resistance was 3. 699; the highest EC50 of isolates collected from Sandong was 698. 39μg/mL, which of the resistance was 334.156. The results present that it was different sensitivities which come from different province in China. There were the difference of sensitive isolates to triadimefon in the same region. The results indicated that there were the resistant isolates to triadimefon in China, and it was different resistance to triadimefon which exsist different province in China.520 random primers were used to amplify some isolates with different sensitive to triadimefon by RAPD analysis of the genome DNA. Twenty-four primers could amplify specific DNA bands in two genome DNA pools, respectively produced by primers OPA5,OPA18,OPC10,OPC12,OPE18,OPH8,OPH11,OPI9,OPI12,OPK3,OPK15,OPL12,OPL 13,OPP9,OPQ3,OPQ13,OPR5,OPS1,OPS11,OPY14,OPY19,OPZ17,S1170.A band of about 500-600bp was obtained with primer OPZ17 amplified the genomic DNA of different sensitive isolates. The band was found from sensitive isolates to triadimenfon, and it was absent in the products from resistant isolates.The fragment OPZ17 was recovered from polyacrylamide gel and cloned in pGEM-T easy vector, then sequenced from two end. According to the sequences, two pairs of specific primers were designed. The RAPD markers was convert to SCAR markers.The different sensitive isolates to triadimefon which were identified from Beijing,Henan, Hebei, Sandong, Gansu and Sichuan in 2004-2005 were amplified with the pair of SCAR primers. The results showed that there were the specific bands could be revealed from 8 of in the 11 sensitive isolates, and no band was detected in the 15 fungicide resistant isolates. This could be convenient tool for monitoring the fungicide resistance of Blumeria graminis f.sp. tritici to triadimefon to detected.