Development Of Anti-satiety Egg-yolk Antibodies To Improve Feed Intake Of Sows During Lactation Period

Feed intake is the cornerstone of animal productivity. The objective of this study is to improve the feed intake of lactation sows by supplying anti-CCK egg-yolk antibodies in diet. Through the research of immune reponse and detecting egg-yolk antibodies titers, an appropriated way of producing anti-CCK egg-yolk antibodies was established. The influence of immunizing CCK and supplying anti-CCK egg-yolk antibodies in diet on hens and lactation sows was evaluated respectively. The results were as follows:1. The method of making compound antigen of unsulfated cholecystokinin octapepited (CCK8ns) was established using BSA as carrier; Compound antigen was also detected through SDS-PAGE and ultraviolet spectrophotometer scaning; We studied on processing character of immune reponse of hens and IgY in egg-yolk. The results indicated that titer of egg-yolk antibodies rose quickly in a week after the second immune and achieved the top at the 3th week (1:2560), then fell slowly at the 9th week. The results confirmed that compound antigen could be improved by using BSA as carrier protein. The results also suggested that using BSA as carrier protein could be suitable to produce higher titer egg yolk antibodies powders.2. Forty laying hens aged 20w were immunized with CCK-bovine serum albumin conjugate, and the IgY induced in egg-yolk was detected by indirect ELISA. We focus on the realationship between the hens' performance and egg-yolk antibody titer. The results indicated that compared with control group, the feed intake of CCK group was 9.25% higher(P<0.01) than control group and average egg weight of CCK group was 4.17% heavier(P<0.01) than control group respectively during the peak of eggs producing. The results confirmed that the feedintake and of laying hens can be improved by immunized with CCK-bovine serum albumin conjugate, and then the performance of laying hens can be improved.3. Forty lactation sows were selected and allotted to four groups each sows was assigned 10 piglets. All diets were formulated to follow NRC recomendation. Thecontrol group A were fed the corn-soybean diet, experimental group B, C and D were fed respectively a diet with 5% fish oil, 1.2% spray-drying egg-yolk powders and 1.2% spray-drying egg-yolk anti-CCK antibodies powders. The influence of anti-CCK egg-yolk antibody on performance of lactation sows was tested. The datas suggested that supplementation of 1.2% spray-drying egg-yolk anti-CCK antibodies powders increased the feed intake 3.8% and litter weight gain 3.9%, and supplementation of 5% fish oil increased the feed intake 3.8% and litter weight gain 3.9%, compared with control group A respectively, and it was seemed that fish oil was superior to egg-yolk anti-CCK antibodies powders in this study but no difference on statistic.