The Dynamic Analysis Of 1 Ay Active HMW-GS Gene From Triticum Dicoccoides K(?)rn. Transferring To Triticum Aestivum L.

In this paper, both distribution of 1Ay high molecular weight glutenin subunit (HMW-GS) and the encoding gene expression from wild emmer (Triticum dicoccoides Korn.. 2n=4x=28, AABB ) were studied. 1Ay subunit was expressed in 55 of 133 wild emmer accessions by SDS-PAGE detection. Molecular biology in combination with cytogenetics was used to examine the F3 progenies of distant hybridization between wild emmer expressing 1Ay subunit and common wheat (T. aestivum L, 2n=6x=42, AABBDD) Chuannong 16. The main results were as follows:The single lines of F3 progenies were detected by SDS-PAGE. The results showed that some had rich electrophoretic patterns of HMW-GS, which had more than six normal patterns. And some had same electrophoretic patterns of HMW-GS, among which there were expression of subunit 1Ay.1Ay gene was amplified through polymorphism chain reaction (PCR) of genomic DNA from wild emmer wheat D1. It was about 1800-1900bp long as same as in Chuannongl6 and the two types of F3 progenies including expressing and no expressing 1Ay subunit. After cloning and sequencing No. 1964, coding and 5'-flanking and 3'-flanking regions of a y-type subunit gene at Glu-Al was obtained. Nucleotide anddeduced amino acid sequence analysis showed that this gene possessed a similar structure as the previously reported 1Ay gene.Cytogenetic analysis showed that chromosome number in roop-tip cells of having same patterns single lines was 28 (which expressed 1Ay). While in the lines which had 42 chromosomes subunit 1 Ay was not expressed.So 1Ay active HMW-GS gene in wild emmer could not express in the genetic background of common wheat. It was clear that the D genome might be responsible for no expression of 1Ay gene in hexaploid wheat.