| OBJECT: To investigate the effect of environment hormone on lepidopteron, studied of 2,4-Dichlorophenol (2,4-DCP) and P-nony-phenol (NP) act on the Bombyx silkworm in vivo, BmN cells, Sf9 cells and silkworm hemopoietic organ (HeO) in vitro. METHODS: Feed the Bombyx siliworm larvae with artificial diet which added the 2,4-DCP and NP, then survey its weight, growth speed, metamorphosis, bionergy and fecundity in strict conditions. Cultured the BmN and Sf9 cells include HeO with 2,4-DCP or NP-added TC-100 medium in vitro, then survey the DNA damage of BmN and the function of HeO, research the cell multiplication of BmN and Sf9 with method of 3-[45-dimethyl (thiazol-l-2-yl)]-2,5adiphenyltetrazolium bromide (MTT). RESULTS: The growth speed, weight, developmental uniformity, bionergy and fecuncity of the Bombyx silkworm descent dramatically as they eat the feed that the concentration of 2,4-DCP over 0.8 mmol L~-1 or NP exceed 0.500 mmol L~-1. But the effect on reproductive capacity vary with gender difference, it's more obvious on the late-stage of development and periods of metamorphosis such as ecolysis, pupation, edosion. There may be cumulative effect inside the Bombyx siliworm. The concentration level of 2,4-DCP and NP in the body and nature environment also have similar functions of environment hormone to silkworm. There have differences in the effect of 2,4-DCP and NP on cell multiplication of BmN and Sf9, so dose the concentration. The IC50 value of BmN and Sf9 cells to 2,4-DCP is about 0.14 mmol·L~-1, to NP about 0.05 mmol·L~-1. If the concentration of NP exceeded 0.128 mmol·L~-1, it can induce the DNA of BmN breakage, nevertheless, it also have concentration effect. The function of the HeO will decline as NP over 0.032 mml·L~-1, as come to 0.128 mmol·L~-1, it lost hemopoietic function completely, only go to death gradually.CONCLUSION: The 2,4-DCP and NP have effects of environment hormone function on Bombyx silkworm, moreover, have the similar influence to other lepidopters. |
