Study On Antagonistic Mechanism And Fermentation Condition Of Trichoderma Koningii

In this study ten species of Trichoderma were morphologically identified. Among them, Trochoderma koningii was systematically studied based on its physiological and biochemical characteristics. Its antagonistic mechanism and biocontrol were studied. In addition, optimizing condition of antibiotics protruded and fermentation on culturing materials were also tested. Results as following:1. For the conidium germination of T. koningii: temperature range is 20-35℃.optimizing temperature is 20-25℃; the thermal death temperature is 71 ℃/10 min;appropriate pH is 3-9; Higher humidity would benefit to the germination;light does not have obvious effection on the germination and hypha growth. Trichoderma koningii has a broad adaptability that enables it to survive and reproduce efficiently at different ecological conditions in our country and that also make it to be used easily in biocontrol of plant diseases.2. Trichoderma kongnii resists pathogens mainly through nutritional competing, hyperparasiting and also producing secondary metabolites. Its secondary metabolites have a heat-stability and have the potential used as biological pesticides.3. Fermentation test for culturing T. koningii on furfural waste dregs: T. koningii would grow and reproduce quickly and efficiently in following conditions: temperature varying from 25 to 20 then to 25 ℃, initial humidity as 22%, inoculating proportion as 8-12%, thickness of culturing material as 20mm. Conidia in furfural dregs could reach 1 × 10~8 conidia/g.4. Field experiment to control cotton take-all caused by Rhizoctonia solani: T. koningii could effectively control the disease. Although different treatments didn't affect the rate of deaded seedlings, they could reduce the rate and also index of the diseased cotton significantly at F0.05 level and F0.01 level, respectively.