The Relation Analysis Of The Twinning And Polymorphism Of FSHβ Gene In Qinchuan Cattle And China Hostein

Qinchuan cattle and China Holstein were used as experimental population. The FSHβgene were studied using PCR-SSCP and analyzed the genetic variation. The relation between genetics markers and the twinning were analyzed. The aim is to explore perfect markers in twinning which can provide theory base on breeding in cattle.the results were follows: 1.The analysis on polymorphism and sequence on the 5'flank of FSHβThe polymorphism were found in PCR amplification region of the premier FSHβP1, FSHβP2 and FSHβP3 in Qinchuan cattle, but only on its of the premier FSHβP2 and FSHβ-P3 in China Holstein . (1) Three genotype AA, BB and AB were found in PCR amplification region of the premier FSHβP1 in Qinchuan cattle, but only one genotype in China Holstein. The polymorphism information content (PIC) values were moderate polymorphism(0.5>P>0.25), which means large variation in the loci. Sequence alignment for the region demonstrated that there were a lot of mutation. At position 65bp added G in the AA　genotype, there were three mutation with the position 73bp(added T),281bp(T→G) and 284bp(G→A). (2) Both normal and mutational genotype were found in PCR amplification region of the premier FSHβP2 in Qinchuan cattle and China Holstein . Sequence for the region demonstrated that there were a lot of mutation. There were one mutation with 642bp(G→A) in normal genotype and two mutation with 642bp(G→A) and 690bp(C→G) in mutational genotype in Qinchuan cattle.There were one mutation with 642(G→A) in normal genotype and two mutation with 642bp(G→A) and 642bp(C→G) in mutational genotype in Qinchuan cattle. (3) Both normal and mutational genotype were found in PCR amplification region of the premier FSHβP3 in Qinchuan cattle and China Holstein . Sequence for the region demonstrated that there were a lot of mutation. There three mutation loci with 1804bp,1805bp,1806bp which three T changed C in normal genotype and mutation genotype in both Qinchuan cattle and China Holstein . In mutational genotype there was one mutational loci with 1700bp(C→T) besides them in Qinchuan cattle, but with 1638bp (added G )besides them in Hostein cow. In Qinchuan cattle and China Holstein at positon 1804bp,1805bp and 1806bp all were C, which research result was not reported by the correlative research.The result was found firstly. 2. The analysis on polymorphism and sequence on exon3 of FSH? The polymorphism were found on exon3 in Qinchuan cattle but China Holstein . Two genotype AA, BB were found exon3 in Qinchuan cattle.The polymorphism information content (PIC) values were moderate polymorphism(0.5>P>0.25), which means large variation in the loci. Sequence for the region demonstrated that there were a lot of mutation. There were two mutational sites in AA genotype with 4314bp and 4338bp which were synonymous mutation, but the mutation at 4378bp and 4380bp result in Threonine to Proline . 3.The relation of the FSH? polymorphism and twinning The polymorphism were analyzed by five premiers in FSH? gene of Qinchuan cattle and China Holstein . The results demonstrated that the mutation frequency in twinning cattle is higher than in monovular cattle of Qinchuan cattle and China Holstein . The result provided liable evidence for bovine prolific reproductive traits. The result is important academic basis and working means to using MAS to study twinning trait in Qinchuan cattle and China Holstein.