Study On The Content Of Th1 Cytokines Affecting Fecundation Rate Of Ewe On The Embryo Transplantation And The Mechanism Of Herbal Medicine Increasing Fecundation Rate

This study was designed to discuss the correlation between the content of Th1 cytokines and the fecundation rate on the embryo transplantation, research the function of nitric oxide (NO) in the embryo implantation and growth, and explain the mechanism of herbal medicine "An Tai San" on promoting the fecundation rate. In this experiment, based on embryo transfer technique, the authors collected plasm and uterine milk from donors and receipts at the time of embryo transplanting and the receipts' plasm was collected after two estrous cycles. The content of cytokines was measured by ELISA and the content of NO was measured through the method of nitric deoxidize enzyme. The results were that the content of Thl cytokines such as TNF-α, IFN-γ, IL-2 in plasm or uterine milk of goats and sheep who were estrous was significantly higher than the normal pregnant goat and sheep (P<0.05),but there was no significant differences between the pregnant receipts and the donors at the time of embryo transplanting(P>0.05). This indicated that if s adverse for embryo implantation and growth to secreted Thl cytokines superfluously. It was important for embryo growth after transplantation to keep the receipts' uterus immune condition conforming to the donors. The content of Th1 cytokines in plasm of pregnant goat after two estrous cycles was lower than that at the time of transplanting. But the defferent wasn't significant (P>0.05). These findings demonstrated that the content of Thl cytokines decreased with the development of pregnancy, which is good to pregnancy. The content of NO in plasm of goat and sheep who were estrous was significantly higher than the normal pregnant goat and sheep (P<0.05).This result showed superfluous NO may do harm to embryos, and led to earlier embryo loss. At the time of embryo transplanting, the changing tendency of the content of NO was conformed to Th1 cytokines in plasm of goat receipts. This result demonstrated there was a correlation between NO and Thl cytokines. Furthermore, we found the NO content of pregnant ewes on pregnant 40 days was significantly higher than the transfer time(P<0.05),but which was significantly lower than estrous ewes at the same time(P<0.05). The content of NO increased with the development of pregnant in order to meet the needs of embryo growth.After embryo transplanting, 20 goat receipts were randomly divided into two groups, including herbal medicine group and comparison group, and 10 goats in each group. So did 14 sheep receipts. Herbal medicine "An Tai San" had been fed on the dose of 100 gram per...