Establishment Of Fingerprinting For Clones In Pinus Massoniana With Microsatellite Markers

One hundred and twenty clones in clonal test stand of Pinus massoniana in WuYi forestry farm, FuJian Province were analysized by microsatellite(SSR) markers. Primers with high polymorphism were screened. DNA fingerprinting for trial materials was established by SSR markers and digitized. At the same time, SSR markers linkaged with growth traits were detected. The main results were summarized as follows: 1. Eight polymorphic primers(PR203, PR4.6, PR011, RPTest1, RPTest11, PtTX2146, PtTX3013 and PtTX4062.) were screened from 140 primers that had been developed in Pinus taeda, Pinus radiata, Pinus sylvestris, Pinus cembra, et al. The number of alleles per locus was from 4 to 12, and the total polymorphic alleles in all loci was 59 which meant per locus had 7.3750 alleles on average. 2. The mean observed heterozygosity (Ho, 0.4538) was less than that of expected heterozygosity (He, 0.5047), but they were not too far. Fit was 0.0965 on average, close to zero, indicating that the clones population was closer to the Hardy –Weinberg equilibrium. All materials in the study were clustered into two groups with SSR primers. One consisted of clones from the generation of the 7th area in west of orchard, the other consisted of clones from that of the 8th, 9th and 10th areas in east of orchard. The later three areas were clustered into three sections, respectively. The clustering was agreed with scattering of clones in the orchard. 3. DNA fingerprinting of 120 clones was established by the molecular fingerprinting combination of 8 primers and was digitized, which made a distinction between all of the clones tested. What's more, 18 clones (numbered 2, 6, 9, 11, 16, 38, 39, 49, 59, 60, 68, 77, 80, 84, 85, 89, 111 and 120.) could be discriminated from others by only one pair of primer. And that others could be differentiated by different combination of fingerpringting. 4. Correlation between seven growth traits(such as height, DBH, stem volume, et al.) and molecular markers was detected. Of all traits, the markers associated with height was not detected, and two markers of PR4.6 and PR011,associated significantly or extremely significantly with other 6 traits was detected. The two markers correlated with branch diameter simultaneously, and accumulative proportion achieved 37.7%, and proportion of PR4.6 was 21.23%. Correlation detection indicated that some QTLs effecting growth traits maybe exist near loci. DNA fingerprinting of the120 clones was established and digitized to differentiate clones, that would be served for forest silviculture. Two markers were detected in correlation analysis which may provide some reference for molecular marker-assisted selection in P. massoniana.