Genetic Analysis And Microsatellite Marker Of Female Sterile Wheat

As one of the major crops in China and all over the world, wheat production plays veryimportant role in world food supply and trade market. Utilization of heterosis is the importantand useful way to promote crop output and quality. One of the obstacle to the utilize wheatheterosis is the approach and technique of the making hybridization. Studying the femalesterile wheat makes it possibly that utilization female sterile wheat mix planting. This studywas through the different populations which are deriver from common wheat and the femalesterile line genetic analysis of the female sterile gene and mark it by microsatellite marker.1. The hybrids F1 and F2 were used to study their male and female fertility when a female sterile line in wheat was one of their parents, the results showed that the female sterile was controlled by one or two pairs recessive genes ostensibly,the expression of the female sterile may be related to two pairs of major genes.2. A study was carried out through the genetic analysis of F2 population which is derived from a common wheat and the female sterile line, the result indicated that the sterile was condition by a pair recessive gene in the cross. The F2 population was used to mark the female sterile gene by microsatellite and BSA (bulked segregant analysis). Among the 1,000 pairs of microsatellite primers, a marker was found polymorphic between the two pools and the two parents. Linkage analysis showed that microsatellite marker cfd36 was linked with the sterile gene, and the genetic distance between the SSR marker cfd36 and the sterile gene was 20.2cM.3. The BC1 population which is derived from a common wheat and the female sterile line was used to mark the female sterile gene by the SSR marker cfd36. Linkage analysis showed that genetic distance between the marker cfd36 and the sterile gene was 13.0cM.