| Twenty Chinese herbs such as Radix Astragali, Radix isatidis and rhizome belamcandae, and amantadine and neutral acriflavine were used in anti-virus experiment in vitro. The Chinese herbs were separately decocted and concentrated by heating to a final concentration of 0.50mg/ml respectively. The concentrations of amantadine and neutral acriflavine were 160μg/ml respectively. All the drug-liquids were sterilized by autoclave and stored at 4℃. In the Vero cell culture experiment in vitro, the cytotoxic influence of different concentrations diluted at multiple proportions was studied to determine the highest non-toxic concentration, and anti-virus mechanism of the effective anti-AIV was studied in three ways: adding medicine to the cultured Vero cell before adding virus, adding virus to the cultured Vero cell before adding medicine, and adding medicme and virus to the cultured Vero cell after they acted for a while. Antiviral activity against H9N2 AIV was estimated in terms of Cytopathic effect, viral titre yields and OD values of MTT colorimetnc method to determine the respective effects of anti-penetration activity against virus, restraint of virus propagation and direct destruction of virus, respectively. The results were as follows.The highest non-toxic concentrations of medicaments were: Radix Astragali, 31.25mg/ml; Lonicera Japonica, 3.90mg/ml; Forsythia suspense, 15.62mg/ml; Radix isatidis, 31.25mg/ml; Rhizome belamcandae, 15.62mg/ml; herba andrographis, 7.81mg/ml; Herba houttuyniae, 15.62mg/ml; Radix et rhizomarhei, 7.81mg/ml; Cyrotomium fortunei, 31.25mg/ml; Cortex phellodendri, 31.25mg/ml; Rhizoma coptidis, 3.90mg/ml; Flos chrysanthemi, 3.90mg/ml; Herba ephedrae, 31.25mg/ml; Herbaschizonepetae, 15.62mg/ml; Radix bupleuri, 15.62mg/ml; Ramulus cinnamomi, 15.62mg/ml; Radix scutellariae, 3.90mg/ml; Cacumen platycladi, 62.5mg/ml; Radix stamonae, 31.25mg/ml; i?a |
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