Studies Of Herbs On Antiviral Activity And Actions Of NDV In Vitro

Newcastle Disease, also known as Asia chicken's plague or fake chicken's plague, can cause an acute and highly infectious disease by Newcastle Disease Virus (NDV) . High mortality rate seriously endangered the poultry industry. Chemical drugs, which have been used, don't work well. Furthermore, because of long synthetical cycle, high cost, great side effects and imperfect immunity, people turned to Chinese herbs. How to develop drugs that are high efficient, less residual and low resistant has become an emergent problem to be solved. By orthogonal experimental design, the anti-NDV activity of traditional six Chinese herbs Folium isatidis, Radix isatidis, Herba houttuyniae, Patrinia villosa juss, Rhizoma polygoni cuspidati, Radix sophorae flavescentis was systematically investigated in chicken embryos. Then scientific combination was carried out. Moreover, the action of Chinese herbal medicine against NDV was studied in chicken embryo fibroblast cell (CEF) in vitro. The best composite was Folium isatidis, Herba houttuyniae, Rhizome polygon cuspidati, Patrinia villosa juss and Radix sophorae flavescentis.Three ways, the first was adding medicine and virus after acted for a while, the second was inoculating virus after adding medicine, the third was inoculating virus before adding medicine, were adopted in chicken embryos and CEF. The anti-NDV action of traditional six Chinese herbs and the better composite was systematically investigated.The result of single medicine in chicken embryos indicated that the maximum non-toxic concentration of Folium isatidis, Radix isatidis, Herba houttuyniae, Patrinia villosa juss was corresponding to l.OOg/ml of raw medicine. That of Radix sophorae flavescentis was corresponding to 0.80g/ml and the Rhizoma polygoni cuspidati was corresponding to 0.30g/ml. In the first way, the other five medicines except Radix sophorae flavescentis against NDV was better. In the second way, the effect of Herba houttuyniae, Patrinia villosa juss, Rhizoma polygoni cuspidati and Radix isatidis to NDV was better, but Radix sophorae flavescentis and Folium isatidis had little effect. In the third way, Radix isatidis, Herba houttuyniae, Patrinia villosa juss were more effective than Folium isatidis, Rhizoma polygoni cuspidate. In short, the effects of Radix isatidis, Patrinia villosa juss and Herba houttuyniae on NDV were higher.The first of three more satisfactory complex composites obtained from the experiment was Folium isatidis, Herba houttuyniae, Rhizoma polygoni cuspidati, Patrinia villosa juss and Radix sophorae flavescentis. The second was Folium isatidis, Radix sophorae flavescentis, Patrinia villosa juss and Radix isatidis. The third was Folium isatidis, Herba houttuyniae,Patrinia villosa juss and Radix isatidis. By using chicken embryos and CEF cell, the composites were tested. The result suggested the maximum non-toxic concentration of all composites was corresponding to 1.00 g/ml of raw medicine.The maximum non-toxic concentration of three better complex composites on CEF was respectively 1/32g/ml, l/64g/ml, l/64g/ml. The first composite had strongest anti-NDV action in the first way. The two formers exhibited obvious antiviral action in the second way.But the three composites against NDV were not effective in the third way. In conclusion, the first composite was the best.In this study, the anti-NDV effective complexes prescriptions of Chinese herbal medicines were screened out by chicken embryos and CEF cell in vitro and the antiviral action of the composites were studied systematically. It provided a theoretic basis for screening out the substance with high anti-NDV activity and prevention and cure on ND.