Nuclear Transportation And In Vitro Maturation Of Goat Oocytes

Three　Experiment　were　conducted　to　determine　the　effect　of　EGF　and　IGF-1　on　in　vitro　maturation　of　goat　oocytes.　Oocytes　were　cultured　in　Oocytes　Maturation　medium　with　EGF　and　IGF-1　alone　or　combinedly　at　different　concentration.　The　results　in　experiment　1　showed　that　there　was　no　significant　different　between　control　group　and　the　group　with　EGF(10μg/L﹑20μg/L﹑30μg/L)　in　maturation　rate.　In　experiment　II　the　maturation　rate　of　goat　oocytes　was　improved　significantly　in　the　groups　with　EGF(20μg/L﹑30μg/L)　(P<0.05),but　not　in　the　group　with　10μg/LEGF.　In　experiment　III　the　maturation　rate　in　the　group　containing　10μg/LEGF　and　20μg/LIGF-1　was　significantly　higher　than　20μg/L　IGF-1　and　10μg/LEGF　groups.The　results　obtained　in　this　study　indicate　that　EGF　and　IGF-1　have　coeffects　on　in　vitro　maturation　of　goat　oocytes.　In　vitro　maturation　oocytes　were　treated　with　CB　or　without　CB　treated.　After　parthenogenetic　oocytes　were　cultured　in　vitro　in　CR1aa　(groupⅠ)or　SOFaa(group　Ⅱ)　medium　supplement　with　10　percent　of　FBS　or　EGS.　The　results　of　this　study　showed　that　in　experimentⅠ　there　was　no　significant　different　between　CB　treated　and　without　CB　treated　group　in　the　ratio　of　cleavage　and　blastocyst　of　parthenogenetic　goat　oocytes;　the　blastocyst　rate　of　embryos　　cultured　in　CR1aa　supplemented　with　10　percent　of　EGS　was　higher　significantly　than　supplemented　with　10　percent　of　FBS.　In　experimentⅡ　there　was　no　significant　different　between　CB　treated　and　without　CB　treated　group　in　cleavage　and　blastocyst　rate　of　parthenogenetic　goat　oocytes;　the　blastocyst　rate　of　parthenogenetic　goat　oocytes　cultured　in　SOFaa　supplemented　with　10　percent　of　EGS　was　higher　significantly　than　supplemented　with　10　percent　of　FBS.　It　showed　that　CB　　was　no　significant　effect　on　the　development　of　goat　parthenogenetic　oocytes.　Culture　medium　supplemented　with　10　percent　of　EGS　has　higher　significantly　effect　than　FBS. SOFaa was better than CR1aa　in　culturing　goat　parthenogenetic　oocytes.　Nuclear　transfer　was　carried　out　with　goat　fetal　fibroblasts　cells　cumulus　granulosa　and　human　fetal　fibroblasts　cells　by　means　of　intracytoplast　injection　and　electro-fusion　respectively.　The　results　of　intracytoplast　injection　showed　that　the　development　capacity　of　cloned　embryos　from　goat　fetal　fibroblasts　cells　and　cumulus　granulosa　cells　were　higher　significantly　than　human　fetal　fibroblasts　cells.　Electro-fusion　result　of　goat　fetal　fibroblasts　cells　was　higher　significantly　than　human　fetal　fibroblasts　cells.　Conclusion:　human　fetal　fibroblasts　cells　had　development　capacity　in　goat　denucleared　oocytes　but　it　was　lower　significantly　than　goat　fetal　fibroblasts　cells　and　　cumulus　granulosa　cells.