Identification Of Quantitative Trait Loci Controlling Anther Length And Seed Dormancy From Common Wild Rice(Oryza Rufipogon Griff.)

Common wild rice (Oryza rufipogon Griff.) is the ancestor of cultivated rice(O. sativa L. ). To identify beneficial genes from common wild rice which have been lost or weakened in cultivated rice is one of important researches on rice resources. In this article, an advanced backcross population(BC3F3) and introgression lines derived from the cross between an accession of common wild rice from Yuanjiang(YJCW rice), Yunnan province, China, as the donor and the elite Indica cultivar 'Teqing'as the recipient, and the cross between an accession of common wild rice from Dongxiang(DXCW rice), Jiangxi province, China, as the donor and the elite Indica cultivar 'Guichao2' as the recipient, respectively, were used to identify genetic factors associated with the anther length and seed dormancy. The main results were as follows:1. A total of 16 QTLs controlling the anther length were identified. Of the 16 QTLs, the QTL near the SSR marker RM260 on the chromosome 11 showed the most positive additve effect, up to 0.24mm.2. A total of 21 QTLs controlling the seed dormancy were detected in this study, distributed on the 12 chromosomes. In the introgression lines derived from the cross between the DXCW rice and 'Guichao2', two QTLs from the DXCW rice near RM25 on the chromosome 8 and RM304 on chromosome 10, respectively, explained 16% and 15% of phenotypic variance, respectively, and the additive value reached -34.27% and -32.76%, respectively, revealling that the allele from O. rufipogon could promote the seed dormancy.Responsive of such loci to a dormancy-broken with a dry heat treatment(55℃, 7days) were further detected. The results showed that 7 QTLs controlling seed dormancy can still be detected , which seemed not to be sensitive to dry heat treatment. The other QTLs which increased the seed dormancy could be easily broken by dry heat treatment.3. In the same storage period, the germination of an advanced backcross population between the YJCW rice and cultivar Teqing has dominantly negative correlation with the inhibitory hormone ABA, dominant positive correlation with promoting hormone GA. While in the other introgression lines, there no notable correlation between seed dormancy and hormone ABA, GA.As the storage period increased, the seed dormancy release was not caused by the increase of GA, but by the relative reduce of ABA.