Cloning And Analysis Of The Salt-tolerant Correlative Gene In Triticum Asetivum L.

It is not only very important to improve crops to research the salt-tolerance in plants, but also is a part of basic researchs. So much attention is paid to the cloning of the salt-tolerant correlative gene in plants. At present, some salt-tolerant correlative gene were cloned along with development of molecule biology, but this is not achieve people' s desires. A cDNA sequence with whole ORF was obtained according as the G09-94 cDNA fragment of cDNA-AFLP by e-clone and is 90% homology to a rice gene whose fuction is unknowned by blastn. The sequencing result confirms the success of e-clone. Northern blot shows the expression in H 8706-34 treated by 0% NaCl, H 8706-34 treated by 1% NaCl, RH 8706-49 treated by 0% NaCl and RH 8706-49 treated by 1% NaCl, but the quantity of expression in RH 8706-49 treated by 1% NaCl is beyond the others. So the gene is named as TaSC ( Triticum asetivum L Salt-tolerant Correlative) and was registered at GenBank as a new gene with the accession number of AY956330. DNA microarrays for gene expression profiles show that the quantity of expression in RH8706-49 leaves in short time is decreased and the one in long time is increased under 1%NaCl stress. Half-quantity RT-PCR was done. The quantity of expression in H8706-34 roots decrease earlier and are the natural quantity later. The result in RH8706-49 is the same of the DNA microarrays for gene expression profiles. TaSC gene can be expressed in roots, leaves, grains, spike, endosperm and so on according to blast ESTs;and it is assigned in 5BL , 5DL , 7DS of wheat chromosomes according to blast Mapped wheat ESTs. The character, structures, location and functions of the TaSC protein were analysed by using many softwares for molecular biology and many professional networks . The protein has four membrane-spanning segments and one signal peptide segment and its pI is 3.96 ; its molecular weight is 15315.76 Da. Furthermore, TaSC protein is localized in the plasma membrane and is 82.2% homology to a predicted membrane protein whose fuction is unknowned and 79.8 % homology to an uncharacterized protein family(UPF0220). It has the site of N-myristoylation, Tyrosine sulfation, Casein kinase II phosphorylation and protein kinase C phosphorylation. So it can be presumed that TaSC gene is a constitute-expressed gene on middle or lower position of the responsed networks under adversity stress in plant and is not tissue-specific. And TaSC protein may play a role in many signal transduction...