Comparative Study On Sex Identifying Ways Of Mammalians

The objective of this study is to develop a rapid and correct ways on sex identifying of mammalian species with polymerse chain reaction (PCR) and immunological assay. The PCR system is developed and verified by using genomic DNA from cows and mouses of known sex. The presence or absence of the SRY (sex-determining region Y) gene region determines sex. Using optimized genomic DNA PCR conditions for SRY primers, genomic DNA from twenty cattles (♀5,♂15)and twenty mice (♀7,♂13)are amplificated. The results are that all cows have bands representing SRY-specific except one and all male mice are the same as cows except two. But all females of these two species don't have. Immunological assay is used for detecting male-specific H-Y antigen on preimplantative embryos of kunming strain mouse. In this experiment, H-Y antigen is detected on preimplantative embryos by cytotoxicity test thereby to determine sex of embryos, 8- cell embryos to blastocyst stage are cultured in DMEM medium added H-Y antiserum. After 24h of culture, embryos were classified as either affected or unaffected. An embryo is classified as affected if there are degeneration of embryo or breakdown of one or more blastorneres. These embryos are H-Y positive (male). Both PCR and immunological assay can be used as methods of sex determination. And they have respective advantages and disadvantages. However, after comparative study, we find that the present sexing technique using PCR is relatively simple ,rapid, while maintaining high degrees of accuracy, and can be widely used in.