| Tetranychus cinnabarinns (Boisduval) is an important agricultural mite pest, infesting over 100 crops or plants grown in the field or greenhouse worldwide.Its management has been depending on insecticides for a long time, which result in its wide and high level resistance to insecticides. Many scholars carried out studies on resistant mechanism of mite at level of biochemistry, but few researches on the molecule resistance mechanism were reported, which set back efficient of mite management and further understanding of resistance evolution. Based on resistance mesuremed to pesticides and esterase electrophoresis of the resistant strain of T.cirmabarinus to abamectin (AbR), studies about cloning and analysis esterase of Txinnabarinus were carried out and the results are as follows.1. Resistance selection.The stock colony of T.cinnabarinus was collected from cowpea in the field in Beibei, and reared under insecticide free conditions in insect-rearing room for 5 years (about 100 generations).This colony was regarded as susceptible strain. The abamectin insecticide root in biology was chosen in this study to deal with T.cinnabarinus, after 52 generation selections, Txinnabarinus developed 11.29 fold resistance to abamectin. Based on the slope value of log-probit regression line, the resistance of Txinnabarinus to abamectin could continue develop to a higher level, but the developmental rate would be slow.2. Esterase electrophoresis of T. cinnabarinusEsterase electrophoresis pictures of susceptible and abamectin resistante strain from Txinnabarinus revealed that they had 4 bands at least with different colors, and E3 esterase had a deeper color than others.In addition, more E3 esterase of AbR strain had a relatively deeper color than that of susceptible strain. This result indicated that the activity of E3 esterase in AbR strain was stronger than in susceptible strain, which had relation with happening resistance to abamectin. |
