The ISSR Molecular Marker Analysis And Cloning Of LFY Homologous Gene Fragment From Syzygium Samarangense Merr.et Perry

Wax apple (syzygium samarangense Merr. et Perry) also known as Samalanga Syzygium, tianwu, Zhuawa roseapple et al. It indigenous from Malaya to the Andaman island, and good in color, smell and taste, is one of several rare fruit trees developed in tropic and southern subtropic rigions of recent years. More than 10 germplasms with excellent characters have been collected by Southern Subtropical Crop Research Institute of CATAS from the world presently, and carried some basic studies accordingly. Because the varities of wax apples mostly named by combining with the place of production and fruit characters, probably brought about synonym and homonym. In additon, a critical technique in cultivation of wax apple is to control the period of flowering, now through pruning, chemical treatment and management for the purpose, but often obtained no obvious effects.According to the above-mentioned two mainly questions, the experiment used black pearl cultivar as the template DNA to obtain the optimum ISSR reaction system of Wax apple. The results showed that the optimum concentrations of components such as Buffer, Mg~2+,dNTPs,Taq DNA polymerase, primer, template DNA in 25μ1 reaction system were lxBuffer(without Mg~2+), 2.0mmol/L,0.2mmol/L, 1.5U, 0.2(Amol/L, 1.0ng/nl, respectively. Furthermore, the addition of 1.6% formamide was propitious to reduce the disturbance.Using the optimum system, 12 varities of wax apples and 2 closely related species, 5. jambos and S.malaccense, were studied of relationship and molecular identification for the first time. A totle of 459 unambiguous bands were got with 18 primers, out of them 435 (94.8%) were polymorphic. Excepted that the Dice coefficient between Haiyin NO.2 and Hainan danhong were 0.882, the others were between 0.347 and 0.811, thus there without any synonym. The dendrogram with UPGMA method showed these accessions were divided into 3 groups: wax apple, S. jambos and S.malaccense. 12 cultivars and varieties of wax apple were further divided into 4 subgroups: (1) Aozhoudanhong;...