Research And Aplication Of The Greenish-Yellow Bud Mutant In Hot Pepper

It had studied the genetic law of leaf color, biological characters, dynamic growth and heterosis of the green-yellow bud mutant in pepper. Researches had also been conducted on the purity identification of hybrids. Selection of cytoplasmic male sterile line and breeding of new varieties using the greenish-yellow character. Results were as follows:1. Yellow-green seedling mutant is a recessive mutant of pepper .The mutant stable yellow-green character, significant genetic character in seedling stage and seedling blight. It can be used in hybrid breeding and purity test of variety.2. The purpose of this experiment was to investigate the biological characteristics and growth trend between two pepper-96-140 yellow bud mutant(96-140YBM)and 96-140.The results showed, 96-140YBM were better not only in plant growth vigor, leaf , plant height and single fruit weight, but also in resistant levels to the phytophthora blight. 96-140YBM has a stable heredity and an obvious genetic mark character in seedling stage and a great value to breeding and hybrid test purity.3. An observation was made on some characteristics of fruit in Fl generation by the 10 multiple combinations between 6 varieties of yellow-green seedling of hot pepper and 3 varieties of sweet pepper. The results indicated fruit length, flesh thickness and fruits/plant appeared heterosis in Fl generation. The over-parent heterosis of fruit length, flesh thickness and fruits/plant were 10.01%, 8.15% and 1.05%.4. The qualification of the hot pepper purity of inbred and hybrid using seedling stage leaf color mark technology and plant test was studied .The results indicated that two test resultsof seedling stage leaf color mark technology and plant test had obvious correlation. Correlation coefficient is 0.9556. So seedling stage leaf color mark technology can be regards as the reliable method of Hot pepper test purity.5. Research and application of cytoplasmic male sterility (CMS) can promote utilization of heterosis in pepper-96-140 yellow bud mutant (96-140YBM) . In this study, the male sterile cytoplasm of hot pepper lines was transferred into the 96-140YBM ones through repeated backcross and individual selection. Four 96-140YBM CMS lines: 96-140YBMA-n with stable male sterility and horticultural performance similar to the original inbred line 96-140YBM were developed, as well as their corresponding maintenance lines 96-140YBMB-n.6. Zhenyan No.8 is a middle-late pepper F₁ Hybrid developed by crossing the self-line YBM96-140-2 with the self-line T9701 selected from Gansu Jiuquan .the fruit of the variety is ox-horn in shape with length of 15cm,shoulder diameter of 4.5cm flesh thickness of 0.40cm,green in color and good quality. Zhenyan No.8 is suitable to be grow in plastic greenhouse in Changjiang reaches and south China, the average weight per fruit is 42.5g,yield is 3545⁴200kg /667m2.