Establishment And Application Of Tissue Culture And Rapid Propagation Protocol For Eucalyptus Dunnii

Eucalypts cold-tolerant species were characterized that the species are cold-tolerant andfast-growth which has some wood and economic value. Cold-tolerant could make plantationareas extend to the north of eucalypts district in China. Eucalyptus dunnii was the rightspecies; it was introduced into China due to its better adaptation to colder climates (includingfrost tolerance) and industrial quality. Nevertheless, its plantation was limited for its seedwere difficult to collect. There were six selective seed-derived trees and a clone from YonganFujian province in this experiment. Stem segments were used as explants to establish aregeneration protocol for it. These seed-derived explants were incubated on a modified MSmedium, supplemented with different concentrations of auxin and cytokinin. Studied axilliarydifferentiation and reproduce, root differentiation. An optimal regeneration system forEucalyptus dunnii was established preliminary. The best cytokinin and its concentration was6-BA0.5mg/L in axilliary differentiation stage, 6-BA0.5mg/L ＋ NAA0.1mg/L and6-BA0.3mg/L＋ NAA0.2mg/L were used for the proliferation stage in turn, in twocompositions clone 25 got a high proliferation coefficient and grew stronger. The optimalmedium for clone 25 to get strong sprout was no hormone modified MS medium. Rootinduction medium containing IBA0.5mg/L was used in one step rooting,rooting rate was62.3%; In the same medium without solidified agent the rooting rate was 55.14%; the optimalIBA concentration for two steps rooting was 3.0mg/L, its rooting rate was 61.57%. Sucroseconcentration in root induction depended on the lignin degree of clone 3, in high degree thebetter range of the Sucrose concentration was 15-25g/L.Macro-element, micro-element,organic and sucrose got some effect on the rooting rate in clone Fujian dunnii, the biggest wasmacro-element, next was micro-element and organic, last was sucrose. In two steps rootingclone 24 got a rooting rate of 54.3%, the best concentration of IBA was 4.0mg/L. Clone 25have been transplanted successfully, the survival percentage was 90%; other three rooted Vclones was not survival. In the same medium five clones grew differently. Clone 24, 3 andFujian dunnii grew well, and rooted on the rooting medium; The proliferation coefficient ofclone 13 was high but no rooting; The hyperhydric of clone 40 was serials; A great quantitycallus emerge from the terminal bud of clone 15. 6 clones were induced from 6 selectedseedlings in 7 clones, another clone was Fujian dunnii. Clone 25 has got rooting andtransplanted successfully; Clone 24, 3 and Fujian dunnii have developed some roots but nottransplanted; Clone 13, 40 and 15 had no rooting.