Transient Expression Of Extrogenic Proteins In The Oocysts Of Eimeria Tenella

The intensive production of many type of poultry and farm animals depends upon kepping animals free from the diseases caused by coccidia, viral and bacterial pathogens. We are developing a novel eukaryotic vector system—transgenic coccidia for multivalent vaccine against major diseases caused by these pathogens of mammals and birds. The result will be a type of vaccine—produing and delivering vaccine which could be delivered orally and interact with the mucosal ,humoral, and cellular compartments of the immune system.The objective of this study is to develop a transient expression system in Eimeria tenella based on the transformation of unsporulated oocysts by using a yellow version of green fluorescent protein as the reporter. Successfully expressed constructs contained sequences of the coding region of the yfp gene.We have determined the conditions and parameters sporulation was delayed 12 hours compared with electroprated untransformed oocysts and half of the transformed oocysts failed to finish sporulation. These results will help to push forward the gene manipulation in coccidia like Eimeria, Isosprora, Tyzzeria, Neospora and Cryptosporidium the life cycle of which spp is not completed efficient in vitro.