| Sweet osmanthus (Osmanthus fragrans Lour) is one of ten traditional famous flowers in China and the excellent landscape trees that used for improving city environments and fragrant economic trees. The major object of this study is to exploit the technique systems of tissue culture and rapid propagation for sweet osmanthus. Using the embryo, fresh shoots and leaves as the explant, the optimal medium and culture programe are studied. The major results as following:1. The concentration of disinfectant mercuric chloride for stems and leaves of sweet osmanthus is 0.10%. The optimizing time of disinfection for stems is 3 minutes and leaves 2 minutes separately.2. The optimizing medium for embryo initial culture was LMc+ BA1.00mg/L+GA1.00 mg/L. The germination ratio was 100% with seedlings fast grow.3. Using the fresh shoots of sweet osmanthus as the explant for initial culture, the best season for selecting material is the last ten-day period of February. The optimizing medium for initial culture is LMc+KT8.00mg/L+NAA0.10mg/L. The germination ratio is 70.70%.4. The optimizing medium for subculture multiplication is LMc+TDZ0.5mg/L+ NAA0.10 mg/L. The average propagation coeffcient per year or yearly isl.6*106.5. The optimizing medium of rooting is l/2MS+NAA2.00mg/L, ratio of rooting is above 95.00%, with strong and quick-grow roots.6. Using humus soil as culture substrate and the base media of l/2LMc to foliar fertilization, the ratio of transplant viability is above 80.00%.7. The optimizing medium for callus induction is LMc+BA0.50mg/L+2, 4-D0.10 mg/L, medium of LMc+BA0.50mg/L+NAA2.00 mg/L is better. Comparing to the culture under light, the culture under dark can form callus more efficiently. |
