Enzymolysis Conditions Of Porcine Blood And The Effects Of Enzymolyzed Porcine Blood Meal On Protein Utilization And Growth Performance Of Piglets

Three experiments were conducted to determine the optimum enzymolysis conditions of porcine blood, to estimate the ileum ammo acid apparent digestibility of enzymolyzed porcine blood meal, and to evaluate the effects of enzymolyzed porcine blood meal on protein utilization and growth performance of early weaned piglets. Experiment 1. The estabolishment of optimum enzymolysis conditions of porcine blood Two L9(43) orthogonal experiments were designed to determine the optimum enzymolysis conditions of porcine blood by papain and As. 1398 neutral protease respectively.Then the optimal combinated conditions of enzymolysis for papain and As. 1398 neutral protease were determined by the design of single factor based on the degree of hydrolysis and the effects of temperature.time and enzyme/substrate ratio on the degree of hydrolysis. The result indicated that the optimum enzymolysis conditions for papain were that enzymolysis temperature was 60C, enzyme/substrate ratio was 6000U/g, enzymolysis time was 4hr. and for As. 1398 neutral protease, enzymolysis temperature 50C,enzyme/substrate ratio 6000U/g, enzymolysis time 6hr. The optimal conditions of enzymolysis for combinated application of papain and As. 1398 neutral protease were that enzyme/substrate ratio was (2000U papain+20001) As. 1398 neutral protease )/g , enzymolysis time was 6hr and temperature was 55 C. Experiment 2. The determination of the apparent ileal digestibilities of amino acids of enzymolyzed porcine blood mealThe design of single factor was adopted to determine the apparent ileal digestibilities of amino acids of enzymolyzed porcine blood meal and unenzymolyzed porcine blood meal for 8.17 0.30kg piglets. Ten DurocxLandrance Large white castrated piglets were allotted randomly to two treatments with five replications per treatment for a 7-d feeding period. All pigs were of a single genetic strain and geographical site of origin.Semi-purified diets were formulated with enzymolyzed porcine blood meal or unenzymolyzed porcine blood meal as the only protein source. Chromium oxide(3g/kg) was included as an indigestible marker. Pigswere allowed ad libitum access to feed and water. After the experiment.the slaughter technique was introduced to determine the apparent ileal digestibilities of amino acids.The result indicated that the ileum amino acid apparent digestibilities of threonine and serine for enzymolyzed porcine blood meal were lower very significantly than that of unenzymolyzed porcine blood meal(p<0.01) and for asparagic acid the apparent ileal digestibility decreased significantly(p<0.05),but for the other amino acids there were no significant differences between the two treatments(p>0.05). The apparent ileal digestibilities of threonine,serine and asparagic acid for enzymolyzed and unenzymolyzed porcine blood meal were 27.55%> 23.76%,68.73% and 63.18%,71.41%,79.63% respectively.Experiment 3. The effects of enzymolyzed porcine blood meal on protein utilization and growth performance of early weaned pigletsThe design of single factor was adopted to determine the effects of enzymolyzed porcine blood meal (EPBM)on protein utilization and growth performance of early weaned piglets compared to.spray-dried porcine plasma protein(SDPP)and unenzymolyzed porcine blood meal(UPBM). There were three treatmenls,in each treatment diets were formulated with EPBM, SDPP and UPBM respevtively and in all treatments soybean meal was included. Eighteen DurocxLandrancexLarge white castrated piglets(7.44 ?0.53kg) were allotted randomly to three treatments with six replications per treatment for a 28-d feeding period. All pigs were of a single genetic strain and geographical site of origin.On the 22nd day of the trial, a 6-d metabolism experiments were conducted.Pigs were allowed ad libitum access to feed and water. The result indicated that the ADG.ADFI.G/F of the diet containing EPBM treatment were lower than that of UPBM and SDPP treatments.Compared to UPBM and SDPP treatments,the ADG,ADFI,G/F of EPBM treatment decreased by 35.0%(p<0.01), 16.