Genome Mapping And Qtl Analysis In Soybean

A scheme of molecular linkage group was constructed with a RIL population from a cross of two cultivated soybeans "Charleston" and "NEAU594" that were derived from the United States and China. Ninty-seven QTL for sixteen important agronomic traits were mapped in the genetic map. The main results are summarized as follows:1. A scheme of molecular genetic map consisted of 20 linkage groups was constructed with the mapping software Mapmaker/EXP3.0 MapChart 2.0, which included 161 SSR markers. It covered approximately 1913.5cM of soybean genome with an average genetic distance of 11.89cM. The number of SSR markers on each linkage group varies from 2 to 28, with the length from 0.4cM to 309.5cM.2. A core population that consisted of 154 recombinant inbred lines was constructed. The lines with the largest number of recombination events were selected to represent all the recombination events.3. The QTL analysis for sixteen important agronomic traits was conducted with the statistical software Windows QTL Cartographer V2.0, including protein content, oil content, protein and oil content, leaf length, leaf width, leaf shape, plant height, 100-seed weight, floss color, flower color, ramify number, pod property, main-stem nodes, growth duration, pods per plant, and seed weight per plant. Ninety-seven QTL for these sixteen traits were detected by the composite interval mapping method.