Cryopreservation Of Marine Animal Gametes And Embryos

In the present paper, to develop simple, reliable and efficient methods for Cryopreservation are reported.Survival rate of 86.5% was obtained in Cryopreservation of the spermatozoa of seabass Lateolabrax japonicus. The prescription of the best antifreeze solution are glycerol 15%+ sucrose 5%+ glucose5%. The motility of fresh spermatozoa descends slowly within the former 2 hours and it is 50.0% at the third hour. The better density of glycerol and DMSO are 15% and 10%. The antifreeze effect of glycerol is better than that of DMSO. The effect of quick thawing is significantly better than that of slow thawing.The ultrastructure of Takifugu rubripes spermatozoa before and after Cryopreservation was studied by means of ultrathin-section TEM technique. The results indicate that cryodamage may cause the membrane of sperm head swelling or disintegrating, the abnormality of the head, the transmutation of mitochondria, the loss of mitochondria.After Cryopreservation of embryo and larvae of manina clam Ruditapes variegata, the results indicate the survival rate of blastula embryo and vlegier are significantly better than that of trochophore and D-shaped larvae(P<0.05); the survival rate of blastula embryo is 42.3% (4.0M DMSO) and the effect of high sucrose density is better than that of low sucrose density with the same DMSO dendity.After Cryopreservation of embryo and larvae of pacific oyster Crassostrea gigas, the results indicate the survival rate of trochophore larvae is 61.1% and significantly better than that of other stage embryo or larvae(P<0.05).The effect of high sucrose density is better than that of low sucrose density with the same DMSO density.