Identification Of Aegilops Tauschii-Triticamcarthlicum Amphiploid And Molecular Marker Of Resistant Genes

Agronomic traits investigation ,cytologic characteristic identification ,resistant investigation to powdery mildew and stripe rust have been carried out inAegilops tauschii(DD)-Triticam carthlicum(AABB) amphiploid(AABBDD). SSRwere employed to identify the resistance genes in F2 population crossed withAm6-4 and Jinan17. The results are showed as follows:1. 30 wheat lines selected from the posterity of Am6 have been investigatedand analysed,the result showes that the 30 lines have excellent exhibition intillers,spikes,resistance to powdery mildew and stripe rust,spike length,etc,they have important value in genetic improvement to common wheat.2. The result of analyse to chromosome configurations showes that the mostlines are stable in cytology,and some have univalent and multivalent, in 11lines number of chromosome is 40 or 41 .The critical result is anastomoticbetween chromosome at root tip cell and chromosome at PMC MⅠ.Theresult of A-PAGE showes that electropherogram of seed gliadins is different inevery germ plasm,and each has its specific band type, so A-PAGE candistinguish the different germ plasm. 3. Genetic analysis and molecular marker were employed to identified a new powdery mildew resistance gene and stripe rust resistance gene in a F2 progeny from the cross Am6-4×Jinan17. The results are showed as follows: Naturely Jinan17 is susceptible to powdery mildew,Am6-4 is resistant to powdery mildew;Among the F2 mapping population, 70 plants showed resistant to powdery mildew and 28 plants showed susceptible to powdery mildew; according to Mendel's lows the trait of resistance was controlled by 5李涛:小麦-粗山羊草双二倍体的性状遗传特点及其抗性基因的分子标记a single dominant gene. 124 wheat microsatellite primer pairs from Dgenome were screened between the resistant parent and the susceptible parent.Sixty eight primers revealed polymorphism between the parents. And 6primers revealed polymorphism between resistant DNA pool and susceptibleDNA pool. The marker Xgwm98 from the chromosome 6D was found to belinked to the new powdery mildew resistance gene with a linkage distance20.42±4.66cM. The marker in the present study provides a starting point forfine mapping of this new powdery mildew resistance gene. Tiaozhong27,29,31,Shuiyuan46 etc are inoculated to AmphidiploidAm6-4 and F2 mapping population,Jinan17etc,the result showes that Am6-4contains resistance gene to stripe rust, Jinan17 is susceptible to stripe rust,and78 plants are resistant to stripe rust,28 plants are susceptible to stripe rust inF2 population. According to Mendel's lows the trait of resistance wascontrolled by a single dominant gene. 124 wheat microsatellite primer pairsfrom D genome were screened between the resistant parent and thesusceptible parent. Sixty eight primers revealed polymorphism between theparents. And 5 primers revealed polymorphism between resistant DNA pooland susceptible DNA pool. The marker Xgdm33 from the chromosome 1Dwas found to be linked to the new stripe rust resistance gene with a linkagedistance 7.53±2.67cM.