| Goose parvovirus (GPV) infection is a highly fatal disease of goslings and Muscovy ducklings under 1 month old .The causative agent of the disease has been classified as a member of the Parvovirus genus of the Parvoviridae, goose parvovirus. The disease is characterized by acute enteritis and high mortality. The outbreak of GPV has been reported in many countries including Europe and China, which result heavy economic losses. This study is to research the molecular characteristics of the virus that popular in 1982.Mean- while,the NS and VP gene were compared with other strains. This study offers a good base for further investigations that including genome function, mutation regulation of genome and pathogeny of the virus et al.1. A field strain of GPV was isolated successfully from goose embryos in this study. We detected the ELD50 of the fifth passage virus(HG5/82) to 12-day-old goose embryos was 10-5.92/0.1ml. No typical clinical signs were observed after inoculation of GPV-HG5/82 strain. We detected the GPV in the anal swab of the inoculated gosling with nested-PCR and sequencing the product of PCR. The nucleotide identity of the PCR product between HG5/82 and GPV strain B was 93.6%.2. Non-structure gene and structure gene of goose parvovirus (GPV) HG5/82 strain were amplified using polymerase chain reaction (PCR) method and cloned into pMD18-T vector, respectively. The two fragments were sequenced and analyzed with reference GPV strains and Muscovy duck parvovirus(MDPV) strains. The results showed that the identity of NS and VP gene between HG5/82 and GPV-YG strain isolated in China is higher than HG5/82 and B that isolated in Hungary. The identity of partial VP3 genes between HG5/82 and MDPV is higher than the identity between GPV and other members of parvovirinae. Furthermore, these results were also proved by the analysis of VP3 gene. This high identity between GPV HG5/82 and MDPV indicated that they would be came from the same ancestor. |
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