| The experiment was carried out to study the effect of sulfur on nitrogen metabolism and nutritional physiological basic of protein and yield formation by the methods of pots and fields. By studying the effect of different levels of sulfur on N accumulation and quality especially protein, supply the theoretical basis for applied fertilizer scientifically on high-quality production of soybean. The paper draws such conclusions: NR activity of soybean leaf has greater correlation with sulfur. S1 and S2 treatments were the better. The influence on NR was different in different period. NR activity was increased significantly at the beginning, then declined in metaphase and anaphase. At the same growth stage, NR activity of different cultivars in the same fertilization was evidence because of genetic characters.The tendency of N accumulation was consistent in nutrient organic of different cultivars and the percentage of N of every nutrient organic was decreasing in the growth stage, but in the seed it was increasing during the formation of the seed. The order of the distribution and percentage of N in different nutrient organic was: Leaf >pod shell>stem. The leaf, stem and pod of NE42 had higher percentage of N and the sustaining period of higher content than other two cultivars; it showed that the high-protein cultivar had the advantage of higher absorption and accumulation with N. The influence of N was significantly by using sulfur fertilizer, but there were different reflection in different genetic soybean. The optimal S treatment was S1 treatment of NE42, S2 treatment of NE163 and S3 treatment of NE1168. The percentage of N was increased in apparatuses such as leaf, stem, pod, which was treated with sulfur, and declined slowly latter. The sulfur stimulated the absorption and utilization of N and reduced environmental contamination.There was more variable in different nutrient organic at the same growth stage. The quantity of ureide in the stem was higher than that of in the leaf at the nutrient growth stage, the order of the quantity of ureide was pod shell >stem >leaf =seed at the reproduction stage. There was great effect of the quantity of ureide in every organic by sulfur. The ureides content in vegetative parts of NE42 were variable. But the reflection of sulfur on each part was different, leave was at R2 stage and R4 stage, stem was at R4, R6 stage, pod shell and seed was only at harvest. The most remarkable sulfur treatment was S1 of NE42, but S3 treatment of NE163 and NE1168.Under the proper sulfur fertilizer condition, the protein content, oil content and protein+ oil content of three soybean cultivars were increased, the S2 treatment was the most significant.The effect of sulfur on gross of amino acid and sulfur-amino acid in seed was remarkable, and it was different with different types of soybean. With sulfur fertilizer increasing, gross of amino acid and sulfur-amino acid in seed of B9395 and HN35 was increasing, but they all declined in NE434.Sulfur could prolong the function period of green leaves, and it was beneficial for the accumulation of dry matter and high yield. S1 treatment was the most remarkable in the experiment. However, leaf area decreased when fertilization treatment above S1 level. So sulfur fertilization was proper applied in practice.The reason which sulfur could increase the yield of soybean was increasing the number of pod and seed of each plant. The reflection of sulfur was different with different kinds of soybean. It was difficult to increase all factors of yield composition together, however one index increasing only was not fitted. In order to realize high yield, every factors should be coordinated with each other. Candidate: Sun Yu Research field: Crop Physiology Supervisor: prof. Zu Wei... |
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