Transformation Of LEA Gene Into Rice Cell And Analysis Of Regenerated Resistant Plantlets

Rice is one of the most important graminaceous crops. Every year virus and pests cause serious damage to rice production. Research on resistance gene engineering of rice has great improvement in past ten years. Recently reports for resistance-transgenic rice are mainly about virus and pests resistance.Chosing mature embryo of "Zhongzuo8593" as material to inducte callus on LS+2,4-D 2.0. Calli of rice were transformed by particle bombardment with plasmid pBY520 containing late embryogenesis abundant protein gene, LEA3, from barley (Hordeum vulgare L.). The bombarded calli were transformed to media CARS+Bialaphos8.0 and KPRS+Bialaphos8.0 for selection. After 2 times selected culture by Bialaphos, three lines resistant calli were obtained. The resistant calli were transformed to LS media with KT1.0mg/l and ZT1.0mg/l and BA1.0mg/l and NAA0.lmg/1 and got three regeneration lines planets two months later.Positive plants were selected quickly by PCR analysis, and the electrophoresis result showed that foreign LEA3 gene integrated into rice genome.Under 0.!% 0.3%0.5% 0.7% 1%NaCl salt stress conditions, positive plants began wilting were late than contradistinguish plants for l0days. All positive plants died later than all contradistinguish plants for 15days. The results showed that the salt tolerance of transgenic rice was increased, the development of damage symptoms (wilting) caused by salt stress delayed.Under 0.002mol/1 0.004mol/1 0.008mol/1 0.0 lmol/1 salicylic acid solution stress conditions, positive plants showed strong OA ability .Though the POD activity of the control is taller than those of No.3 and No.4, but No.2 has the strongest ability to OA. The result of physiological analysis confirmed the positive plants have strong resistance to salt and water deficit than the controls.