Changes Of Reproduction & Procreation Hormone Of Muscovy Duck And Total RNA Lsolation From Pituitary

Broodincss is one of the primary factors to influence the reproduction ability of the poultry. In order to promote the reproduction of the muscovy ducks, some research was done to get the rule of the reproduction and the change rule of the procreation hormone and biochemistry index of the blood during the reproduction period, to analyse the incretion mechanism of the muscovy' s broodiness. And study on method for total RNA isolating from pituitary of muscovy duck.The abdominal palpation method was applied to the further research of the muscovy' s laying and brooding rule. The results showed that: (l)The laying time of the muscovy duck can be devided into the first and the second laying period. The first laying period can last about 22 to 25 weeks. Then came molting period which would last about 10 to 12 weeks. After that was the second laying period which would last about 20 to 22 weeks. Then the muscovy ducks would stop laying. The average laying frequency of the muscovy' s first laying period was 0.48, 0.46 in the second period. But the discrepancy is not salience. One muscovy duck would lay 156. 57 eggs in total on average during the whole laying period, of which 213 was the the most, while 73 was the least. (2)The laying climax time was different between summer and winter. It was at 4 o' clock in the winter morning, while 5 o' clock in summer. What' s more, the laying climax time was longer in summer. The temperature could give much influence to the laying ability. The average laying rate was 27.48% in winter, 51.50% in summer. (3)The individual difference of the brooding period was very big, the shortest of which was only 30 days, while the longest one was 106 days. In the whole reproduction period of the muscovy duck, the twice brooding rate was 13. 33%, and the once broodingrate was 25. 19%. Only 61. 48% of them had no brooding period. But their laying paused frequently. The rate of the brooding ducks between the first and the second laying period was nearly the same. They were 18.25% and 17.18% respectively. (4)The average age of the first laying time was positively correlated markedly with the weight of the duck. And the quotiety was 0. 198(P<0.05); But it was negatively correlated weakly with the production of the first laying period.And the quotiety was -0.015. The weight when the duck started to lay was negatively correlated very markedly with the production of the first laying period. The quotiety was - 0. 272(p<0. 01). And it was positively correlated very markedly with the the length of the brooding period (p<0. 01). The change of the weight during the brooding period was positively correlated very markedly with the length of the brooding period. And the quotiety was 0. 973 (p<0. 01). (5)The laying amount was negatively correlated very markedly with the length of the brooding period(p<0. 01). The laying time of the first egg of the brooding ducks came earlier than the non-brooding duck, and also the laying climax time was shorter; the laying-rate declined quickly, while the non-brooding ducks could keep a high laying-rate (36. 37%) and a longer laying-climax period. The study on the change rule of the duck' s procreation-hormone concentration during brooding period by radioactivity immunity analysis (RIA), the results showed that:(l)The average concentration of plasm PRL during brooding period was higher than non-brooding period (p<0. 01); (2)The concentration of the plasm LH of broodniess and non-broodniess was different markedly, and the concentration of the plasm LH of non-brooding period was higher markedly than the broodniess period(p<0. 01); (3)The average concentration of the plasm in non-brooding period was higher than brooding period(p<0. 01); (4)The change trend of the plasm PRL was contrary to the LH concentration during brooding and non-brooding period, the change trend of the plasm Ez was consistent to the LH concentration in every broodniess phase. (5) The concentration of the plasm PRL was negatively correlated markedly with the plasm LH, the quotiety was-0.923; and the concentrati...