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Study On Genetic Coadaptability Of Structure Loci Between Tan Sheep And Small-Tailed Han Sheep

Posted on:2005-05-30Degree:MasterType:Thesis
Country:ChinaCandidate:L DuFull Text:PDF
GTID:2133360125452746Subject:Animal breeding and genetics and breeding
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Using simple random sampling in typical colony method,60 Small-Tailed Han Sheep and 73 Tan Sheep were sampled respectively in their central area of habitat Liang Shan county, Jin Ning city of Shan Dong Province and Yan Chi county of NingXia Hui Nationality Autonomous Region.Every individuals had respective frequencies of appearance characters.Then on experiment ,starch gel electropherisis was used to detect the variations of 16 blood enzyme and other protein structural loci, computing the gene frequencies on every polymer locus and going to the research on genetic co-adaptability.At the same time, it also detected the polymorphism of "microsatellite" DNA site which distributed on 6 different chorosomes. The results were as follows:1. Through researching on the genetic markers, such as the appearance characte the body measurements of the two populations, we found that there has some difference of different sex between different breeds in different age; At the same time, the low reliability of estimated values existed in the rare appearance character of the two populations. However, the general investigated results corresponds with the results of Sheep and Goat Breeds in China .2. Among the 16 detected blood enzyme and other protein structural loci, Small-Tailed Han Sheep had 10 polymorphism loci, the every dominant gene were X-p? Dia?Ary-Es?Lap?AlpB?Cat?Hb?ME?Gc?Tf? However, Tan Sheep had 11 polymorphism loci, the every dominant gene were Ly?X-p+ Dia?Ary-Es? LapB? AlpB? Cat8 ?Hb?MEF? Gc?Tf? The propotion of genetic polymorphism of Small-Tailed Han Sheep was 81.25%, but that of Tan Sheep was 84.375%.3. The 3 best suitable loci were selected from 16 structural loci to carry out thegenetic coadatatility research. Then, adding the Hu sheep and Tong sheep's information which were used by Sun wei formerly. Through detecting the genetic equilibrium. We can find : On Hb , Hu sheep and Tong sheep were in the most disequilibrium state (P<0.01) ,on the other two loci they were in equilibrium state (P>.05) . However, Small-Tailed Han Sheep and Tan Sheep were in equilibrium state on the 3 loci (P>0.05) .4. Counting the every two loci of 3 combinations of the four sheep populations. The results were as follows: As to Small-Tailed Han Sheep, Hb- P -Me was in the most disequilibrium state (P<0.01 ) , the two other were in linkage equilibrium state (P>.05) . However, Tan Sheep were in linkage equlibrium on the 3 combination. Hu sheep and Tong sheep, the two combinations were in the most disequilibrium except the linkage equlibrium of Cat-ME.5. Using the classical Cluster to analysize the genetic coadaptability data on the 3 loci of Small-Tailed Han Sheep?Tan Sheep?Hu Sheep and Tan Sheep, its result were as follows: Hu Sheep was closer to Tong Sheep in their phylogenetic relationship, so they combine to a cluster. Small-Tailed Han Sheep and Tan Sheep combine to a cluster. At last, the four sheep were combined to a general cluster.6. Using classical cluster to analysize the 20 indexes of the four sheep through the genetic coadaptability coefficient?appearance character and ecological character. The results were as follows: At first, Small-Tailed Han Sheep was combined with Tong sheep as a cluster. Then, they combine with Tan Sheep as another cluster. At last, Hu Sheep was combined to them.7. After collecting the data of native and foreign sheep populations, the discriminant for fussy pattern was used to disclose the two sheep's systemic status and discriminant of extraction. Tan Sheep and Small-Tailed Han Sheep are thought to belong to "Mongolia sheep" group, it correspond with the historical records exactly.
Keywords/Search Tags:Tan Sheep, Small-Tailed Han Sheep:Structural loci, Genetic coadaptatility, Genetic approach degree, Appearance character, Ecological character
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