Cloning Of Potato PTOM1 Full-length CDNA And Construction Of Trangenic Potato Plants Expressing Varied Forms Of PTOM1

RNA viruses encompass about two-third of all virus genera and inelude numerous important pathogens. Host factors play important roles in the viral RNA replication and transportation in host cells. Here, we eport the cloning of a host factor PTOM1 gene from potato with high omology with Arabdopsis thaliania TOM1 that supports the replication f tobacco mosaic virus. The full-length cDNA of PTOM1, 1257 bp in ength, encodes a deduced 256 a.a. protein. To identify the function of TOM1 during the viral infection of the potato host, a series of plasmids ere constructed: (1) antisense PTOM1 cDNA in plant-transforming ector pBI121; (2) PTOM1 cDNA-based RNAi fragments in lant-transforming vector pBI121; (3) antisense PTOM1 gene fragments in pBI121 which derived from genomic potato DNA and (4) Antisense TOM1 cDNA into virus-transforming vector PTVOO. These constructs ere used to generate transgenic potato plants. The results of PCR emonstrated that the target genes were integrated into the genome ofpotato plants. An efficient potato transformation system with transformation rate higher than 65%, and winthin a time frame of 5-7 weeks to obtain a transgenic potato plantlet was developped.