| In this paper, trials were conducted to study the degradability of lignin in bagasse and rice straw treated by mutational white-rot fungi and Aspergillus niger through solid-state fermentation, selecting bagasse and straw as experimental objects. And the digestibility of fermented feed was also study through in vitro digest trial by simulating rumen environment with buffalo rumen liquid.Experiment 1 was designed with L9 (34) of the orthogonal table to investigate the effect of the ratio of solid and liquid, the ferment temperature ,ferment time, and pH value on degradability of lignin and cellulose in bagasse and rice straw. The sequence influenced by the four factors was the ferment time, ratio of solid and liquid, temperature and the pH value in order, the best condition was that the ferment time, ratio of solid and liquid, temperature and the pH value were 30 days, 1:3, 30C and 6.5 respectively. The highest value of degradability of white-rot fungito lignin in bagasse and rice straw were 11.29%and 13.49%,and those of degradability of Aspergillus niger to cellulose in bagasse and rice straw were 6.59%and 6.20%, whereas the highest value of degradability of combined microorganism to lignin in bagasse and rice straw were 8.61% and 18.78%.Experiment 2: A digest trial was conducted to study the in vitrodigestibility of fermented feed by simulating rumen environment withbuffalo rumen liquid .The results shown that the digestibility of fibercarbohydrate of bagasse and rice straw treated with microorganism weresignificantly higher than those of the untreated group. The lignin in vitrodigestibility of bagasse and rice straw treated by white-rot fungi,Aspergillus Niger combined were 5.56%, 6.35%, 3.59%, 3.52%,4.04%, 5.31%, which were increased by 175.25% (P<0.05, 123.59%(P<0.05, 77.72%(P<0.05, 23.94%(P<0.05),100%(P<0.05)and 86.97%(P<0.05) respectively compared to the untreated group.
|
PDF Full Text Request