The Molecular Assay And Assessment Of Agronomic Traits Of Disease-Resistant Genes Transgenic Rice

Taking super rice restoring lines-9311 and E32, into which 4 disease-resistant genes, rch10, rac22, b-rip and B -glu, have been transformed, sterile line- Pei'ai 64s and their hybrids as experimental materials, molecular assay has been done and agronomic traits have been assessed. The conclusions are drawn out as follows:1 PCR and Dot-southern blotting analysis showed that B -glu gene had integrated into the genomes of transgenic 9311 E32 and their hybrids. On the basis of theory of genetic linkage, rchl0 rac22 b-rip genes had also integrated into the genomes of transgenic 9311 E32 and their hybrids.2 The genomes of T2 generation of transgenic 9311 and E32 were integrated with B -glu gene suggested that foreign genes could be inherited stably. B -glu gene could be detected in Pei'ai64s/transgenic E32 showed that foreign gene could be transferred by ordinary crossing.3 By PCR , the B -glu gene positive plants of T2 generation of transgenic 9311 and E32 were screened out.4 The assessment of agronomic traits showed that the growth duration of transgenic E32. Pei'ai64s/ transgenic E32, Pei'ai64s/transgenic 9311 was extremely significantly shorter than their controls, and the plant height of transgenic E32, Pei'ai64s/ transgenic E32, Pei'ai64s/ transgenic 9311 was significantly or extremely significantly shorter than their controls, and the value of some economic characters of transgenic 9311, E32 and their hybrids was also lower than their controls. However, the plants whose agronomic traits were good can be gained.5 The method of making biotin-label probe was discussed. It was concluded that the probe made by PCR had some advantages that density was high, sign was intensive and it's no need of making fragments having the same sequence of detected DNA.