Development And Characterization Of Triticum Aestivum-Hordeum Californicum Alien Chromosome Lines

T. aestivum c.v. Chinese spring (CS)-H. californicum amphiloid was introduced and confirmed by chromosome C-banding and genomic in situ hybridization, and a standard C-band pattern of H. californicum chromosomes was obtained. CS-H. californicum amphiloid was crossed to CS, then backcrossed and self-fertilized. Among the BC2F2 progenies, five disomic addition lines, eleven monosomic addition lines, four dimonosomic addition lines, two telosomic addition lines, one monosomic substitution line and nineteen multiple addition lines (including chromosome substitution and translocation) were obtained by a combination of multiple techniques, such as root tip cell (RTC) chromosome C-banding, PMCMI configuration analysis, fluorescence in situ hybridization (FISH) and simple sequence repeat(SSR). SSR analysis was also used for rapid identification of alien chromosome lines and characterization of homoeologous groups of the added H. californicum chromosome. Preliminary results indicated that H. californicum chromosome H2 had homoeologous relationship with wheat group-7 chromosome and chromosome H6 had homoeologous relationship with wheat chromosome 4B. The amphiploid and the identified alien chromosome lines were further indentified for wheat scab and powdery mildew resistance. The results indicated that CS-H. californicum amphiploid was high good resistant to powdery mildew and better scab resistance than CS. and should be a new genetic resource for wheat breeding on powdery mildew resistance.