Constructing Of DNA Molecular Marker Linkage Map And Mapping Of Qualitative And Quantative Traits In Upland Cotton

Two multiple-markers lines (T582 and T586) of upland cotton (Gossypium hirsutum L. ) were used as parents to produce a F2 population consisting of 120 individual plants, which were used to construct a molecular linkage map. On the basis of observing the qualitative and quantative traits of the map population, Two types of DNA molecular marker methods, simple sequence repeals (SSR) and amplified fragment length polymorphism (AFLP), were used to construct a linkage map of molecular markers and to map genes of agronomic characters. The main results are as follows:1. 30 SSR markers and 69 AFLP markers were polymorphic between T582 and T586.2. All the polymorphic molecular markers and morphological markers were used to construct linkage map with Mapmaker3.0. This linkage map was composed of 73 markers including 24 SSR markers, 41 AFLP markers and 8 morphological markers. These 73 markers were assigned to 18 linkage groups.3.According to the information of morphological markers in chromosomes, 5 linkage groups were assigned to the chromosomes.4. 12 quantitive characters were analyzed with QTL1. 0 and 11 QTLs of 8 quantitive characters were detected.This study first tagged three genes (cl,, fg, Lc1) of mophorlogical traits with DNA molecular markers and set up a relationship between classical genetic map and DNA molecular linkage map. It will benefit the studies of genetics and breeding in cotton.