| Yellow-seeded rape (Brassica napus L.) is characterized with thinner seedcoat, higher oil and protein content and lower fiber compared with black ones under the same genetic background . Many researchers obtained the same conclusion through their studies that the content of protein and oil content is negatively related with the luster of the testa..On the other hand, the heredity of the testa color is not unstable.Based on this,we tried to understand the metabolism pathway containing the pigment,protein and carbohydrate through chemistry regulation using three pairs of near-isogenic lines of black- and yellow-seeded rape as experiment materials.The results are as follows:1 .Characteristic of lipid synthesis in rape: The dynamic variation of activity of many enzymes such as SDH,IDH,ILY and oil content were measured and the difference between the two types rape was analysed. The oil content in the yellow- was not significantly different from the black-seeded lines at the early development stage. With the maturation of the seeds,the oil bagan to accumulate quickly, what's more, the accumulation in the yellow- was faster than that of black ones.After maturation,the oil content in the yellow- was obviously higher than that in yellow-ones. The activity of the enzymes (SDH,IDH,ILY) was low at the early period of development, then they increased and reached the maxium in 31 days after flowering. The changes in the content of SDH,IDH and ILY was very obvious between yellow- and black-seeded in seed and the difference was up to significant level. However,the difference among variety was not obvious. The difference of SDH,1DH and ILY in seed was significant level when seed was in maturity between yellow- and black-seeded in seed. Correlation analysis between one group of enzymes(SDH,IDH and ILY) and oil content was done.2 The correlation coefficient between oil content and enzymes including SDH and ILY was positive,it is no positive between oil content and IDH,which suggested that SDH and ILY affected oil content of rapeseed.While the activity of SDH enzyme was repressed ,the oil content was decrease. We utilized FHH to repress oil synthesize ,the oil content also was drop.Citric acide promoted oil synthesize.3 Relationship between oil content and protein was as followsrthe oil content was increase but protein decreas after treatment of rape with protein(HLN) and oil regulaters(LMS). :the oil content was decrease but protein increas after treatment of rape with oil regulaters(FHH). Correlation analysis between oil content and protein indicated that oil content was negative correlation withprotein.4 Relationship between oil content and sugar was as follows:the oil content and sugar was delining after treatment of rape with FHH and NPO respectively,while treated with LMS.the oil content and sugar was increase.it showed no significant correlation by variance analysis.5 Effect of treatment with chemical regulations during different time on rapeseed.the time was 7days and 1day before mature and after harvest.the plant or seed were treated with 7chemical regulaters.The results are as follows:The effect of chemical regulaters on oil content of rapeseed:special chemical regulators.such as citric acide.sodium sulfite and Vc could improve oil content of rapeseed, the mixture of 2% sodium sulfite and Ig.L'1 Vc had the best effect among 7 groups of chemical regulators in lday before gaining,which improve oil content of rapeseed . All those regulators in the experiment could improve oil content of threshing seed of yellow-seeded rapeseed,but 1% citric acide had the best effect among 7 groups of chemical regulators.The effect of chemical regulaters on yellow-seeded degree of rapeseed: the mixture of 2% sodium sulfite and 1g.L-1 Vc had the best effect among 7 groups of chemical regulators in lday before gaining,which improve yellow-seeded degree of yellow-seeded rapeseed. All those regulators in the experiment could improve yellow-seeded degree of yellow-seeded rapeseed of threshin...
|
PDF Full Text Request