Studies On Induceing Plantlet Regeneration Of Resistant Mutants And Determination Resistance To Verticillium Dahliae In Eggplant

In vitro screening of resistant mutant was carried out by biotechnology of plant tissue culture ,with the toxtion filtrate of Verticillium Dahliae as stress factor.Some mutant resistance to Verticillium Dahliae were obtained and produced regenerated plants ,and then the regenerated plants were determined resistance. The experiment used the step by step method.The resistance of calli to Verticillium Dahliae toxion could be improved by increasing the colony times,and the suitable time was 4.With improving concertration of Verticillium Dahliae toxion,the ratio of calli survival was decreased.The result showed that the effect of two eggplant regeneration was best on the differentiation medium of F1 (MS medium+NAA1.0mg/kg+BA5.0mg/kg) through analyzing several factors,such as the differentiation ratio of sprout,the upgrowth of sprout, the time of forming bud.The toxion of pathogen could inhibit the differentiation ratios of plant. The ratio of induction root on medium of G1 was higer than G2 or G3. we idenfied the resistance to Verticillium Dahliae using toxion and conidispore inoculation,and found the resistance level of regenerated plantlets by screening in vitro were obvious higher than control plantlets .After measuring the physiological and biochemical characterization of regenerated planlets,the result was that increased of activities of Peroxidase(POD), Polyphenoloxidase(PPO),and Phenylalanine ammonialyase(PAL) compared with control planlets.