The Cloning Of TTOM1 Gene From Tomato And Construction Of Its RNA Silencing Vectors

It is known that host factors are required for viruses to replicate and/or maintain in the host cells. However, only a few host factors with clear in vivo function have been identified. A tomato gene, TTOMl, which supports the replication of cucumber mosaic virus, was cloned. The full-length genomic DNA of TTOMl, 6726 bp in length, contains 11 extrons and 10 introns. To identify the function of TTOMl during the viral infection of the plant host, we constructed RNA silencing binary vectors (including RNAi vectors and viral vectors) which are used to generate TTOMl RNA silencing transgenic tomato plants of TTOMl. Three transgenetic lines of tomato were obtained.