| In this experiment,sat-monosome,diploid and sat-trisome of Chinese Cabbage with different characteristics were chosen as the materials.In order to find out how the sat-chromosome additioning or droping affected the Chinese Cabbage growing,Several index of their functional leaves were measurd by the use of the British PP-systems corporation, such as net photosynthetic rate(Pn),diurnal change of Pn,the response curve of PAR to Pn ,chlorophyll content,Hill reaction activity,soluble protein,Rubisco activity,leaf anatomical structure and the ascorbic acid,soluble sugar and dry/fresh weight at their harvesting stages.And some theoritic foundation and practical guidance would be received through this study and helped to realize high-photosynthetic efficiency breeding using the aneuploid plant. The main results are as follows:1. Compard to diploid,the additioning or dropping of sat-chromosome had a negtive effect on the photosynthetic rate(Pn).But the Pn of sat-trisome was higher than that of sat-monosome at the reproductive growing stage .However, at the nutritive growing stage the Pn of sat-monosome was higher than that of sat-trisome.2. The curves of diurnal change of Pn of three lines were similar,all showed typical two peaks at the nutritive growing stage.But at the reproductive growing stage ,the decline scope of Pn of monosome and trisome was smaller than diploid at noon.monosome showed an unconspicuous "noon sleep".3. At the reproductive growing stage,the light compensation point(LCP)and the light saturation point(LSP)of sat-monosome is 67.7μmol·m-2·s-1 and 1397μmol·m-2·s-1; 60.7μmol·m-2·s-1 and 1370μmol·m-2·s-1 in sat-trisome; 48.4μmol·m-2·s-1and 12.85μmol·m-2·s-1 in diploid. At the nutritive growing stage ,The LCP and LSP of sat-monosome is 87.7μmol·m-2·s-1 and 905μmol·m-2·s-1 ;83.9μmol·m-2·s-1 and 867.5μmol·m-2·s-1 in sat-trisome; 59. 7μmol·m-2·s-1 and 927.5 μmol·m-2·s-1 in diploid.4. The additioning or dropping of sat-chromosome had a positive effect on chlorophyll content and a negative effect on chla/b and boosted the degree of thylakoid stacking.chla/b can be used as a primary choice character for high photosynthetic efficiency breeding materials.5. The additioning or dropping of sat-chromosome had a negtive effect on Hill reaction activity and Rubisco activity,a little effect on soluble protein . The decline of Hill reaction activity and Rubisco activity were the direct factors resulting in low photosynthetic rate of sat-monosome and sat-trisome.6. The additioning or dropping of sat-chromosome affected the leaf anatomicalstructure.More tightly arranged mesophyll cell and less porosity were observed in them and the ratio of palisade tissue to spongy tissue went up.The density and size of stoma had also been affected.7. The additioning or dropping of sat-chromosome had some effects on the nutritive quality of Chinese Cabbage,a positive effect on Vc content and a negtive effect on soluble sugar and dry weight. |
