Studies Of Nitric Oxide (NO) On The Germination, Growth And The Relevant Mechanisms In Oryza Sativa

The seeds of Oryza sativa (Zhongnong9095) were treated by different concentrations of SNP, the donor of nitric oxide (NO), to underatand: First, the status of the germination of the root and bud, the growth of the root. Second, the number and size of the root-tip cells were statistically analyzed by using the paraffin section technique and correlative software. Third, the expression of cdc2-Osl and cdc2-Os2 genes, that control the transition of G1-S during cell cycle, by Northern-blotting.The results indicated that the growth and development of the Oryza sativa were changed obviously with the treatment of different concentrations of SNP. It was showed that: at the lower concentration of SNP (≤10uM), the root grew faster than the control, but slower at the higher concentration (>100uM); when [SNP] ≤50uM, the germination of the root was as much as the control, but lower down about 30% when [SNP] ≥100uM; the germination of bud was nearly the same as that of root; the number of the cells were lower gradually with the rise of the concentration of SNP, but there was no obviously difference of the area of the cells after alalysis by using the paraffin section technique and correlative software; the expression of cdc2-Osl and cdc2-Os2 were inhibited when [SNP] ≥100μM.From these data, we can conclude that NO can impact the growth and germination of the root by inhibite the cell division, especially the transition of G1-S.