| Alopecurus Aequalis (S.) is one of the noxious weed in the field, which widely distributed in china, Japanese, kerora, and Turkey in Asian. Now it becomes a troublesome weed in our country, especially in the middle and lower region of Yangzi River. It mainly distributed in the field of wheat and oil rape. The Alopecurus Aequalis (S.) In the rape field was investigated in this paper, the dormancy, germination of the seed and biology of the anthesis of the species were studied, and the resistance to ACCase-inhibitor in Alopecurus Aequalis populations were identified. The modal anatomy, physiology and biochemistry, molecular biology was employed to discuss the mechanism of resistance. The results are as follows:There is exists 1-3 month's dormancy for seeds of Alopecurus Aequalis, so it is necessary to find a rapid method to break the seed dormancy to make it germinate consistently. The result showed that it is related to the substance and the time of the treatment. The seed need to be immersed in GA3 with 800ppm concentration about 5 days, and the germination percentage will be enhanced to 80%. Treated with different preservation ways, storage in soil with immersed water is benefit to get through the summer that is one of the reasons of its occurrence easily in the field of rice-rape or rice-wheat.The bioassay to whole individual, to seeds and to pollen were used to detect the resistance of Alopecurus aequalis populations to haloxyfop-R, quizolofop-R , the results indicated that Alopecurus aequalis from the rape field without herbicide treatment is susceptible, while that population from the rape field in LiYang county where it has being used with the haloxyfop-R about 10 years has high resistance. The resistance level is: LYR>HFRI> HFRII> JXRI > JXRII.The cross- resistance in Alopecurus aequalis populations was also identified. The results showed that population of Alopecurus aequalis from the rape field without herbicide treatment is still susceptible to sethoxydim, and those populations of JXRII, JXRIfrom Jiangning whose resistance have been detected resistance to haloxyfop-R are high resistant to sethoxydim, while populations from LYR whose resistance are high to haloxyfop, but is susceptible to sethoxydim. This result suggested that different herbicide-resistant modes exist mAlopecurus aequalis populations.There was remarkable difference to Electrical conductivity, MDA, GSH, POD, chK car, soluble protein and sugar between the resistant and susceptible populations (JXRII, JJSII) after treated by haloxyfop-R ,This suggested the herbicide strongly affected the physiological and biochemical aspects of the susceptible biotype.After treated with herbicide for 6d, the Electrical conductivity of leaf in JJSII increased rapidly. The content of MDA rose four times in contrasted to the control, this implied membrane-lipid peroxidation was intensified. The content of chal and car decreased 1.6and2 times respectively; Two days later after treatment, GSH become lower, while the GSH from JXRII was higher contrasted to the control .the soluble sugar were increased both the susceptible and resistant biotype. But the former increased higher than the later, the activity of POD from JXRII increased slowly than that population from JJSII.The Bi-directional Allele-specific PCR was used to detect the base in molecular level of the resistance to sethoxydim. Only JXRII, JXRI can be amplified the SOObp specific-fragment, while others amplified the 780bp fragment. It is concluded that there probably exist the ACCase mutation within JXRII, JXRI. The base in molecular fragement level of the resistance to haloxyfop-R was studied, only LYR can be amplified the 490bp specific-fragment, while others amplified the 1100bp fragment which further verify there are many mutation points in the ACCase. At the same time, It also illuminates the target sites are different between AOPP and CHD.
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