Studies On Indirect Hemagglutination Diagnostic Preparation And Reverse Indirect Hemagglutination Diagnostic Preparation Of FMD

A strain of O type FMDV isolated from Inner Mongolia district was used in this study. After being consentrated and purified, the virus was used to immune animals and high-immunify-level serum was obtained ; Then reverse indirect hemagglutination diagnostic preparation was obtained after sheep red blood cell was sensitized with the purifying the high-immunify-level serum by salt-outing and chromatographying. The indirect hemagglutination diagnostic preparation was obtained by sensitization of sheep red blood cell with purified FMDVThe activity and speciality of the diagnostic preparation were tested . The result showed that the activity and speciality of the reverse indirect hemagglutination diagnostic preparation is good. It could only agglutinate the same type positive antibody and could be used to detect FMD antibody . The result of comparision of sensitivity between our diagnostic preparation and the diagnostic preparation produce by Lan Zhou Vererinary Institute showed that our diagnostic preparation had a higher and more stable sensitivity than the reverse indirect agglutination diagnostic preparation of FMD produce by Lan Zhou Vererinary Institut. Therefore , it can be used to FMD antibody detection . The speciality of indirect hemagglutination diagnostic preparation is good, but the result of comparision of sensitivity of our diagnostic preparation is lower than the diagnostic preparation produces by Lan Zhou Vererinary Institute.