Effect Of Fat Sources And Chromium Picolinate On Lipid Metabolism In Broiler Chickens

The objective of the research was to evaluate the effects of dietary supplementation with fat sources and chromium picolinate on the lipid metabolism of broiler chickens.216 1-day-old broilers were assigned at random to 6 groups with 3 replicates.2(0 and Img/kg chromium picolinate) X 3(beef tallow,soybean oil and fish oil)treatment were used.The broiler were raised for 8 weeks.The apparent availability of dietary nutrients in broiler chicks were obtained during 15-17 days old,and component of muscle were assayed.The ADG, F/G, PDAF, L/B, and TP, GLU, BUN, TG, TC, VLDL.LDL, HDL in serum were detected at interval Hdays.Two chickens of each groups were killed quickly in 29d,and got a piece of liver.RT-PCR was applied to dectect gene expression of FAS.ACC, HMG-CoA reductase.The results were as follows: (1) The apparent availability of fish oil was higher than soybean oil and beef tallow (P<0.05), and there was a tendency of increasing the apparent availability of crude protein (CP) by chromium; (2) Addition of the different fat sources influenced abdominal fat deposition slightly,and fish oil increased weight of liver significantly (P<0.05). Dietary chromium supplement improved growth,increased F/G and decreased abdominal fat content, but difference was not significant(P>0.05); (3) Fish oil in dietary decreased serum TG, TC, and HDL concentration, but difference level was not coincidence. Supplement of chromium decreased BUN, GLU and TG, on the contrary, TP and HDL concentration were risen, similarly, there was not the sameness of effect degree; (4) Fish oil especially decreased the content of cholesterol. The content of protein in carcass was improved by chromium, and triglyceride and cholesterol decreased; (5) Dietary fish oil reduced by 50.35%,40.35%, 81.49% the hepatic abundance of FAS, ACC, HMG-CoA reductase, compared with beef tallow. Fish oil ,rich in polyunsaturated fatty acid, was more effective than soybean oil; (6) Effects of 1mg/kg chromium picoiinate on abundance of FAS and HMG-CoA reductase mRNAs is obvious, reduced by34.82% and 48.26%, compared with no chromium picoiinate group. However the change of abundance of ACC mRNAs reduced by only 3.87%.